污渍
免疫印迹
定量分析(化学)
计算机科学
化学
生物
生物化学
色谱法
基因
作者
Lakshmi Pillai‐Kastoori,Amy Schutz‐Geschwender,Jeff A. Harford
标识
DOI:10.1016/j.ab.2020.113608
摘要
Attaining true quantitative data from WB requires that all the players involved in the procedure are quality controlled including the user. Appropriate protein extraction method, electrophoresis, and transfer of proteins, immunodetection of blotted protein by antibodies, and the ultimate step of imaging and analyzing the data is nothing short of a symphony. Like with any other technology in life-sciences research, Western blotting can produce erroneous and irreproducible data. We provide a systematic approach to generate quantitative data from Western blot experiments that incorporates critical validation steps to identify and minimize sources of error and variability throughout the Western blot process.
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