Jurkat细胞
细胞凋亡
热休克蛋白
细胞生长
分子生物学
流式细胞术
细胞培养
膜联蛋白
生物
细胞
T细胞
细胞生物学
癌症研究
免疫学
生物化学
免疫系统
基因
遗传学
作者
Ge F,Rong Guo,Wencong Tian,Fengcai Gao,Ling Sun,Zhongxing Jiang
出处
期刊:PubMed
日期:2017-08-01
卷期号:25 (4): 1011-1015
被引量:1
标识
DOI:10.7534/j.issn.1009-2137.2017.04.009
摘要
To explore the effect of heat shock protein 90(HSP90) inhibitor 17-DMAG, an inhibitor specific for heat shock protein 90, on the proliferation and apoptosis of acute lymphocytic leukemia cell lines Jurkat.Jurkat cells were collected, then were treated with 17-DMAG. The expression of HSP90 was examined by semi-quantitative RT-PCR analysis, the effect of 17-DMAG on cell proliferation were detected by using WST, and cell apoptosis were detected by using flow cytometry with Annexin V/PI double stenining.After Jurkat cells were treated with different concentrations of 17-DMAG for 48 hours, the HSP90 mRNA expression decreased significantly in dose dependent manner (r=0.9530, P<0.01). The IC50 was 3.17 mmol/L when the Jurkat cells were treated with 17-DMAG for 48 h; after treating Jurkat cell with 17-DMAG, the cell proliferation was inhibited(r=0.9903, P< 0.01), the cell apoptosis was increased in dose dependent manner (r=0.9876, P<0.01).17-DMAG can inhibit the Jurkat cell proliferation and induce the Jurkat cell apoptosis.
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