茜素红
颅面
解剖
颅骨
软骨
生物
染色
形态发生
病理
医学
遗传学
基因
作者
Joshua B. Studdert,Heidi Bildsoe,V. Pragathi Masamsetti,Patrick Tam
出处
期刊:Methods in molecular biology
日期:2021-12-16
卷期号:: 43-50
被引量:3
标识
DOI:10.1007/978-1-0716-1847-9_4
摘要
Craniofacial morphogenesis is underpinned by orchestrated growth and form-shaping activity of skeletal and soft tissues in the head and face. Disruptions during development can lead to dysmorphology of the skull, jaw, and the pharyngeal structures. Developmental disorders can be investigated in animal models to elucidate the molecular and cellular consequences of the morphogenetic defects. A first step in determining the disruption in the development of the head and face is to analyze the phenotypic features of the skeletal tissues. Examination of the anatomy of bones and cartilage over time and space will identify structural defects of head structures and guide follow-up analysis of the molecular and cellular attributes associated with the defects. Here we describe a protocol to simultaneously visualize the cartilage and bone elements by Alcian blue and Alizarin red staining, respectively, of wholemount specimens in mouse models.
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