Competitive Interactions Between Microbial Siderophores and Humic-Like Binding Sites in European Shelf Sea Waters

铁载体 化学 环境化学 海水 质谱法 电感耦合等离子体质谱法 需氧菌素 溶解有机碳 电喷雾 贻贝 色谱法 生物 生物化学 生态学 基因 大肠杆菌 肠杆菌科
作者
Martha Gledhill,Kechen Zhu,Dagmara Rusiecka,Eric P. Achterberg
出处
期刊:Frontiers in Marine Science [Frontiers Media]
卷期号:9 被引量:12
标识
DOI:10.3389/fmars.2022.855009
摘要

Siderophores are low molecular weight high affinity iron chelates found at low concentrations in seawater. In this study we determined the total concentrations and identities of siderophores in extracts isolated from a shelf sea environment on the Northwest European shelf by high performance liquid chromatography coupled to high resolution inductively coupled plasma mass spectrometry (ICP-MS) in parallel to high resolution electrospray ionisation mass spectrometry (ESI-MS). We identified a total of 24 different siderophores in our samples via metal isotope profiling of masses detected by ESI-MS. Twenty three of the identified siderophores could be assigned to three siderophore families – ferrioxamines, amphibactins and marinobactins. In contrast, only 12 peaks could be resolved in iron chromatograms obtained via ICP-MS analysis. Comparison of results obtained by the two mass spectrometry detectors showed that neither method was able to detect and identify all siderophores present in the samples on its own. We assessed the impact of our observed total siderophore concentrations on iron speciation by calculating the distribution of iron species as a function of total siderophore concentrations at different iron concentrations representative of our study area. We considered competition for iron between siderophores, a humic like dissolved organic matter (DOM) fraction and hydroxide ions by combining an ion-pair model with a non-ideal competitive interaction (NICA)-Donnan model. We found that the overall impact of siderophores on iron biogeochemistry is low at concentrations of siderophore <100 pmol L -1 , and that the dominant iron species present at siderophore concentrations of the order of a few tens of pmol L -1 will be iron bound to the humic like DOM fraction. Furthermore the heterogeneity of binding sites in the humic like DOM fraction means that other binding sites present in organic matter could be effective competitors for siderophores, especially at low iron concentrations. Our findings highlight the importance of binding site heterogeneity when considering the influence of different iron binding groups on iron speciation in the marine environment.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Rjy完成签到 ,获得积分10
1秒前
性感母蟑螂完成签到 ,获得积分10
7秒前
ruochenzu完成签到,获得积分10
9秒前
陈尹蓝完成签到 ,获得积分10
10秒前
天道酬勤完成签到,获得积分10
12秒前
14秒前
仁爱的谷南完成签到,获得积分10
14秒前
雯雯完成签到 ,获得积分10
16秒前
一路有你完成签到 ,获得积分10
16秒前
17秒前
ruochenzu发布了新的文献求助10
17秒前
19秒前
wanghao完成签到 ,获得积分10
20秒前
图图发布了新的文献求助10
20秒前
十三完成签到 ,获得积分10
20秒前
聪慧芷巧完成签到,获得积分10
21秒前
米博士完成签到,获得积分10
22秒前
研友_VZGVzn完成签到,获得积分10
23秒前
Cheung2121发布了新的文献求助30
24秒前
黄芩完成签到 ,获得积分10
25秒前
42秒前
秋半梦完成签到,获得积分10
44秒前
李爱国应助科研通管家采纳,获得10
47秒前
科研通AI2S应助科研通管家采纳,获得10
47秒前
搜集达人应助科研通管家采纳,获得10
47秒前
打地鼠工人完成签到,获得积分10
48秒前
彩色半烟完成签到,获得积分10
50秒前
量子星尘发布了新的文献求助10
54秒前
Ning完成签到,获得积分10
57秒前
图图完成签到,获得积分10
57秒前
勤奋的灯完成签到 ,获得积分10
57秒前
ludong_0完成签到,获得积分10
57秒前
Asumita完成签到,获得积分10
58秒前
双青豆完成签到 ,获得积分10
58秒前
1分钟前
fxy完成签到 ,获得积分10
1分钟前
合适的幻然完成签到,获得积分10
1分钟前
沐雨汐完成签到,获得积分10
1分钟前
1分钟前
1分钟前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038066
求助须知:如何正确求助?哪些是违规求助? 3575779
关于积分的说明 11373801
捐赠科研通 3305584
什么是DOI,文献DOI怎么找? 1819239
邀请新用户注册赠送积分活动 892655
科研通“疑难数据库(出版商)”最低求助积分说明 815022