已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

Competitive Interactions Between Microbial Siderophores and Humic-Like Binding Sites in European Shelf Sea Waters

铁载体 化学 环境化学 海水 质谱法 电感耦合等离子体质谱法 需氧菌素 溶解有机碳 电喷雾 贻贝 色谱法 生物 生物化学 生态学 基因 大肠杆菌 肠杆菌科
作者
Martha Gledhill,Kechen Zhu,Dagmara Rusiecka,Eric P. Achterberg
出处
期刊:Frontiers in Marine Science [Frontiers Media]
卷期号:9 被引量:12
标识
DOI:10.3389/fmars.2022.855009
摘要

Siderophores are low molecular weight high affinity iron chelates found at low concentrations in seawater. In this study we determined the total concentrations and identities of siderophores in extracts isolated from a shelf sea environment on the Northwest European shelf by high performance liquid chromatography coupled to high resolution inductively coupled plasma mass spectrometry (ICP-MS) in parallel to high resolution electrospray ionisation mass spectrometry (ESI-MS). We identified a total of 24 different siderophores in our samples via metal isotope profiling of masses detected by ESI-MS. Twenty three of the identified siderophores could be assigned to three siderophore families – ferrioxamines, amphibactins and marinobactins. In contrast, only 12 peaks could be resolved in iron chromatograms obtained via ICP-MS analysis. Comparison of results obtained by the two mass spectrometry detectors showed that neither method was able to detect and identify all siderophores present in the samples on its own. We assessed the impact of our observed total siderophore concentrations on iron speciation by calculating the distribution of iron species as a function of total siderophore concentrations at different iron concentrations representative of our study area. We considered competition for iron between siderophores, a humic like dissolved organic matter (DOM) fraction and hydroxide ions by combining an ion-pair model with a non-ideal competitive interaction (NICA)-Donnan model. We found that the overall impact of siderophores on iron biogeochemistry is low at concentrations of siderophore <100 pmol L -1 , and that the dominant iron species present at siderophore concentrations of the order of a few tens of pmol L -1 will be iron bound to the humic like DOM fraction. Furthermore the heterogeneity of binding sites in the humic like DOM fraction means that other binding sites present in organic matter could be effective competitors for siderophores, especially at low iron concentrations. Our findings highlight the importance of binding site heterogeneity when considering the influence of different iron binding groups on iron speciation in the marine environment.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
3秒前
mxm12138发布了新的文献求助30
4秒前
liuyue发布了新的文献求助10
5秒前
9秒前
9秒前
Orange应助Lidocaine采纳,获得10
10秒前
10秒前
TRY发布了新的文献求助10
11秒前
Fezz完成签到 ,获得积分10
13秒前
16秒前
17秒前
17秒前
20秒前
21秒前
领导范儿应助俏皮的白柏采纳,获得10
25秒前
26秒前
26秒前
Lidocaine发布了新的文献求助10
26秒前
wiky完成签到,获得积分10
30秒前
大个应助ma采纳,获得10
30秒前
希特勒完成签到,获得积分20
30秒前
zhongu发布了新的文献求助10
30秒前
30秒前
万能图书馆应助HongY采纳,获得10
32秒前
32秒前
Lidocaine完成签到,获得积分10
32秒前
希特勒发布了新的文献求助10
35秒前
37秒前
37秒前
1218完成签到 ,获得积分10
40秒前
45秒前
46秒前
苏和杨发布了新的文献求助10
48秒前
nicenice发布了新的文献求助10
48秒前
48秒前
49秒前
50秒前
万能图书馆应助称心嫣娆采纳,获得10
51秒前
52秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989811
求助须知:如何正确求助?哪些是违规求助? 3531927
关于积分的说明 11255560
捐赠科研通 3270706
什么是DOI,文献DOI怎么找? 1805035
邀请新用户注册赠送积分活动 882181
科研通“疑难数据库(出版商)”最低求助积分说明 809190