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Metabolomics reveals the mechanism of tetracycline biodegradation by a Sphingobacterium mizutaii S121

四环素 生物降解 化学 微生物降解 代谢途径 拉伤 降级(电信) 质谱法 色谱法 生物化学 细菌 微生物学 新陈代谢 微生物 生物 抗生素 有机化学 电信 解剖 计算机科学 遗传学
作者
Hao Tan,Delong Kong,Qingqing Li,Yiqing Zhou,Xu Jiang,Zhiye Wang,Rebecca E. Parales,Zhiyong Ruan
出处
期刊:Environmental Pollution [Elsevier]
卷期号:305: 119299-119299 被引量:41
标识
DOI:10.1016/j.envpol.2022.119299
摘要

Contamination by tetracycline residues has adverse influences on the environment and is considered a pressing issue. Biodegradation is regarded as a promising way to treat tetracycline residues in the environment. Here, strain Sphingobacterium mizutaii S121, which could degrade 20 mg/L tetracycline completely within 5 days, was isolated from contaminated soil. The characteristics of tetracycline degradation by strain S121 were investigated under various culture conditions. Response surface methodology was used to predict the maximum tetracycline degradation ratio, which can be obtained under the following conditions: 31.36 °C, pH of 7.15, and inoculum volume of 5.5% (v/v). Furthermore, extracellular tetracycline biodegradation products and intracellular metabolic pathways of S121 were detected by ultraperformance liquid chromatography-quadrupole-time-of-flight-mass spectrometry (UPLC-Q-TOF-MS) and UHPLC-quadrupole electrospray (QE)-MS, respectively. The results identified eight possible degradation products, and three putative degradation pathways were proposed. In addition, exposure to tetracycline produced significant influences on metabolic pathways such as pyrimidine, purine, taurine and hypotaurine metabolism and lysine degradation. Consequently, the intracellular metabolic pathway response of S121 in the presence of tetracycline was proposed. These findings are presented for the first time, which will facilitate a comprehensive understanding of the mechanism of tetracycline degradation. Moreover, strain S121 can be a promising bacterium for tetracycline bioremediation.
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