电离辐射
细胞生长
镜头(地质)
放射生物学
细胞
辐射
辐照
上皮
化学
核医学
癌症研究
生物
细胞生物学
病理
光学
医学
内科学
放射治疗
物理
生物化学
核物理学
作者
Stephen Barnard,Alice Uwineza,Alexia A. Kalligeraki,Roisin McCarron,Felicia Kruse,M. Brai,Roy A. Quinlan
出处
期刊:Radiation Research
[BioOne (Radiation Research Society)]
日期:2021-05-13
卷期号:197 (1)
被引量:10
标识
DOI:10.1667/rade-20-00294.1
摘要
Lens epithelial cell proliferation and differentiation are naturally well regulated and controlled, a characteristic essential for lens structure, symmetry and function. The effect of ionizing radiation on lens epithelial cell proliferation has been demonstrated in previous studies at high acute doses, but the effect of dose and dose rate on proliferation has not yet been considered. In this work, mice received single acute doses of 0.5, 1 and 2 Gy of radiation, at dose rates of 0.063 and 0.3 Gy/min. Eye lenses were isolated postirradiation at 30 min up until 14 days and flat-mounted. Then, cell proliferation rates were determined using biomarker Ki67. As expected, radiation increased cell proliferation 2 and 24 h postirradiation transiently (undetectable 14 days postirradiation) and was dose dependent (changes were very significant at 2 Gy; P = 0.008). A dose-rate effect did not reach significance in this study (P = 0.054). However, dose rate and lens epithelial cell region showed significant interactions (P < 0.001). These observations further our mechanistic understanding of how the lens responds to radiation.
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