化学
过氧化物酶
纳米颗粒
抗氧化剂
检出限
组合化学
色谱法
酶
纳米技术
有机化学
材料科学
作者
Yanmin Liang,Runli Li,Haiguo Sun,Jie Dan,Zehui Su,Yi Kang,Qiuping Zhang,Shuo Shi,Jianlong Wang,Wentao Zhang
标识
DOI:10.1016/j.snb.2022.131541
摘要
Natural peroxidase composed of protein and metallic cofactor suffers from deactivation in harsh environment. Learning from the natural structure of active site in peroxidase, in this work, natural melanin nanoparticles from cuttlefish were facilely functionalized with Fe3+ to construct peroxidase-like nanozyme, showing high stability and activity. In the presence of H2O2, Fe-melanin nanoparticles (Fe-NMPs) catalyzes the formation of·OH from H2O2, converting colorless 3,3,5,5-Tetramethylbenzidine (TMB) into blue oxidized TMB (ox-TMB). When the antioxidants were added into the system, ox-TMB will be reduced to colorless TMB, thus allowing the detection of total antioxidant capacity. The results show that the lowest detection limit of AA in Fe-NMPs is 12.61 μM and the detection range is 5–250 µm. The test results for AA were similar to the sample instructions for beverages and fruits, validating the feasibility of using Fe-NMPs for the actual total antioxidant capacity determination. This study provides a broader perspective for fabricating foodborne nanozyme for related applications.
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