纳米载体
聚乙烯亚胺
介孔二氧化硅
基因传递
小干扰RNA
基因沉默
纳米颗粒
生物相容性
化学
纳米技术
生物物理学
材料科学
介孔材料
遗传增强
转染
生物
生物化学
基因
有机化学
催化作用
作者
Qing Chang,Chenghao Liu,Zhiquan Xie,Qingfeng Shu,Yijun Xie,Qianqian Su,Xiaoyong Deng
出处
期刊:ChemNanoMat
[Wiley]
日期:2022-01-21
卷期号:8 (3)
被引量:7
标识
DOI:10.1002/cnma.202100453
摘要
Abstract Small interfering RNA (siRNA) for gene therapy has attracted great attention. However, due to easy degradation and inaccessibility to the cells, the gene silencing efficiency is not always satisfactory. To overcome this hurdle, developing an excellent nanocarrier to deliver siRNA into cancer cells is crucial. Fluorescent mesoporous silica nanoparticles are considered to be a superb nanocarrier for siRNA delivery systems because of its negligible toxicity and good biocompatibility. Herein, we developed an efficient fluorescent ultrasmall mesoporous silica nanocarrier for siRNA loading and delivery. The ultrasmall nanocarriers with polyethylenimine coating have a uniform size of about 10 nm. We find that the amino groups on polyethylenimine can protect siRNA from being degraded. Based on the fluorescence observation, we show that polyethylenimine‐coated mesoporous silica nanocarrier loaded with siRNA can be uptake by 293T cells. Importantly, we also prove that siRNA can be delivered into 293T cells by our nanocarrier and subsequently inhibit the expression of related genes. The study provides insights into the development of nonviral fluorescence nanocarriers for siRNA delivery, which have important implications for targeted cancer therapy.
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