Non-specific effects of a CINNAMATE-4-HYDROXYLASE inhibitor on auxin homeostasis

生长素 生物化学 内生 化学 分解代谢抑制 生物 细胞生物学 基因 突变体
作者
Ilias El Houari,Petr Klíma,Alexandra Baekelandt,Paul Staswick,Veselina Uzunova,Charo I. del Genio,Ward Steenackers,Petre I. Dobrev,Ondřej Novák,Richard Napier,Jan Petrášek,Dirk Inzé,Wout Boerjan,Bartel Vanholme
标识
DOI:10.1101/2021.10.27.466161
摘要

ABSTRACT Chemical inhibitors are often implemented for the functional characterization of genes to overcome the limitations associated with genetic approaches. Although being a powerful tool, off-target effects of these inhibitors are easily overlooked in a complex biological setting. Here we illustrate the implications of such secondary effects by focusing on piperonylic acid (PA), an inhibitor of CINNAMATE-4-HYDROXYLASE (C4H) that is often used to investigate the involvement of lignin during plant growth and development. When supplied to plants, we found that PA is recognized as a substrate by GRETCHEN HAGEN 3.6 (GH3.6), an amido synthetase involved in the formation of the auxin catabolite indole-3-acetic acid (IAA)-Asp. By competing for the same enzyme, PA interferes with auxin conjugation, resulting in an increase in cellular auxin concentrations. These increased auxin levels likely further contribute to an increase in adventitious rooting previously observed upon PA-treatment. Despite the focus on GH3.6 in this report, PA is conjugated by an array of enzymes and their subsequent reduced activity on native substrates could potentially affect a whole set of physiological processes in the plant. We conclude that surrogate occupation of the endogenous conjugation machinery in the plant by exogenous compounds is likely a more general phenomenon that is rarely considered in pharmacological studies. Our results hereby provide an important basis for future reference in studies using chemical inhibitors.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
八八九九九1完成签到,获得积分10
4秒前
优雅的千雁完成签到,获得积分10
5秒前
没用的三轮完成签到,获得积分10
6秒前
8秒前
你我的共同完成签到 ,获得积分10
10秒前
mayberichard完成签到,获得积分10
12秒前
风信子deon01完成签到,获得积分10
15秒前
Pt-SACs发布了新的文献求助10
15秒前
香山叶正红完成签到 ,获得积分10
16秒前
wsg完成签到,获得积分10
16秒前
serendipity完成签到 ,获得积分10
18秒前
爆米花应助Yong-AI-BUPT采纳,获得10
19秒前
Pt-SACs完成签到,获得积分10
24秒前
刚子完成签到 ,获得积分10
25秒前
nicky完成签到 ,获得积分10
25秒前
果冻橙完成签到,获得积分10
27秒前
27秒前
田様应助科研通管家采纳,获得10
27秒前
Xiaoxiao应助科研通管家采纳,获得10
27秒前
思源应助科研通管家采纳,获得10
27秒前
28秒前
30秒前
火星人完成签到 ,获得积分10
31秒前
四叶草完成签到 ,获得积分10
32秒前
干净的沛蓝完成签到,获得积分10
32秒前
Yong-AI-BUPT发布了新的文献求助10
34秒前
琥1发布了新的文献求助10
34秒前
livra1058完成签到,获得积分10
34秒前
朴素的紫安完成签到 ,获得积分10
34秒前
火星上小土豆完成签到 ,获得积分10
35秒前
Silole完成签到,获得积分10
36秒前
Yong-AI-BUPT完成签到,获得积分10
43秒前
júpiter完成签到,获得积分10
44秒前
qiaoxi完成签到,获得积分10
44秒前
44秒前
cuddly完成签到 ,获得积分10
45秒前
mzrrong完成签到 ,获得积分10
46秒前
酢浆草小熊完成签到 ,获得积分10
47秒前
mcl发布了新的文献求助10
49秒前
chun完成签到 ,获得积分10
50秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
Immigrant Incorporation in East Asian Democracies 600
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3968578
求助须知:如何正确求助?哪些是违规求助? 3513391
关于积分的说明 11167428
捐赠科研通 3248822
什么是DOI,文献DOI怎么找? 1794499
邀请新用户注册赠送积分活动 875116
科研通“疑难数据库(出版商)”最低求助积分说明 804664