原细胞
合成生物学
纳米技术
人工细胞
生物
活细胞
生命系统
埃布先生
变形虫(属)
凝聚
生物
生物物理学
化学
细胞生物学
细胞骨架
膜
细胞
材料科学
生态学
计算生物学
生物化学
作者
Can Xu,Nicolas F. Martin,Mei Li,Stephen Mann
出处
期刊:Research Square - Research Square
日期:2021-11-10
被引量:20
标识
DOI:10.21203/rs.3.rs-959347/v1
摘要
Abstract Advancing the spontaneous bottom-up construction of artificial cells with high organisational complexity and diverse functionality remains an unresolved issue at the interface between living and non-living matter. To address this challenge, a living material assembly process based on the capture and on-site processing of spatially segregated bacterial colonies within individual coacervate micro-droplets is developed for the endogenous construction of membrane-bounded, molecularly crowded, compositionally, structurally and morphologically complex synthetic cells. The bacteriogenic protocells inherit diverse biological components, exhibit multi-functional cytomimetic properties and can be endogenously remodelled to include a spatially partitioned DNA/histone nucleus-like condensate, membranized water vacuoles and a self-supporting 3D network of F-actin proto-cytoskeletal filaments. The ensemble is biochemically energized by self-sustainable ATP production derived from implanted live E. coli cells to produce a cellular bionic system with amoeba-like external morphology and integrated life-like properties. Our results demonstrate a novel bacteriogenic strategy for the bottom-up construction of functional protoliving micro-devices and provide opportunities for the fabrication of new synthetic cell modules and augmented living/synthetic cell constructs with potential applications in engineered synthetic biology and biotechnology.
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