鞘糖脂
乳糖神经酰胺
自然杀伤性T细胞
生物
糖脂
体外
球三糖神经酰胺
分子生物学
免疫学
生物化学
细胞生物学
化学
医学
内科学
疾病
细胞毒性T细胞
法布里病
作者
Dapeng Zhou,Chengfeng Xia,Peng George Wang,Zhiyuan Li,Wenpeng Zhang,Guanghui Ni,Jiaxi Cheng
出处
期刊:Methods in molecular biology
日期:2021-01-01
卷期号:: 13-25
标识
DOI:10.1007/978-1-0716-1775-5_2
摘要
Glycosphingolipids (GSL) are natural ligands of NKT cells. Several laboratories have reported the in vitro activity of isoglobotriosylceramide (iGb3) in stimulating NKT cells. However, the knockout mice of iGb3 synthase showed no deficiency in development and function of NKT cells. There is a lack of knowledge on the genetics of redundant natural glycosphingolipid ligands. We have identified additional glycosphingolipid with stimulatory activity to NKT cells, including fucosyl lactosylceramide (H antigen). Here we describe the procedures to generate mice with deficiencies in Fut1, Fut2, and Sec1 genes to deplete H antigen through BAC engineering for the generation of ES cell-targeting construct, as well as the mice with deficiency of both blood group H-GSL ligand and isoglobotriosylceramide.
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