孵化器
微流控
实验室晶片
炸薯条
膜
硅片
纳米技术
扩散
生物相容性材料
材料科学
硅
化学
工程类
生物医学工程
电气工程
光电子学
物理
生物化学
微生物学
生物
热力学
作者
Frank Bunge,Sander van den Driesche,Michael J. Vellekoop
出处
期刊:Sensors
[MDPI AG]
日期:2017-07-10
卷期号:17 (7): 1603-1603
被引量:25
摘要
Lab-on-a-Chip (LoC) applications for the long-term analysis of mammalian cells are still very rare due to the lack of convenient cell cultivation devices. The difficulties are the integration of suitable supply structures, the need of expensive equipment like an incubator and sophisticated pumps as well as the choice of material. The presented device is made out of hard, but non-cytotoxic materials (silicon and glass) and contains two vertical arranged membranes out of hydrogel. The porous membranes are used to separate the culture chamber from two supply channels for gases and nutrients. The cells are fed continuously by diffusion through the membranes without the need of an incubator and low requirements on the supply of medium to the assembly. The diffusion of oxygen is modelled in order to find the optimal dimensions of the chamber. The chip is connected via 3D-printed holders to the macroscopic world. The holders are coated with Parlyene C to ensure that only biocompatible materials are in contact with the culture medium. The experiments with MDCK-cells show the successful seeding inside the chip, culturing and passaging. Consequently, the presented platform is a step towards Lab-on-a-Chip applications that require long-term cultivation of mammalian cells.
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