化学
泌尿系统
致癌物
尿
加合物
脱氧腺苷
代谢组学
串联质谱法
生物标志物
色谱法
质谱法
生物化学
DNA
内科学
医学
有机化学
作者
Yanhui Feng,Saide Wang,Hui Wang,Ying Peng,Jiang Zheng
标识
DOI:10.1021/acs.jafc.7b05186
摘要
Methyleugenol (ME), a natural ingredient of several herbs and spices used in the human diet, is hepatocarcinogenic in rodents. Following metabolic activation to the reactive carbocation intermediate, ME can bind covalently to DNA, which is directly associated with its carcinogenicity. In this work, a non-invasive approach to determine ME exposure was established by monitoring the urinary N6-(methylisoeugenol-3′-yl)-2′-deoxyadenosine (ME-dA) adduct. The developed method entails liquid–liquid extraction enrichment of urinary ME-dA, incorporation of deuterated ME-dA as an internal standard, and analysis by liquid chromatography coupled tandem mass spectrometry. Male rats (10–12 weeks, 180–200 g) were treated (p.o.) with ME, and ME-dA was excreted in urine in a dose- and time-dependent manner. The non-invasive approach enabled us to successfully determine exposure to ME-containing herbs and spices. These results suggest that ME-dA can potentially serve as an effective biomarker of ME exposure in rats. It is expected that the developed approach of detecting urinary ME-dA will facilitate the investigation of ME carcinogenesis.
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