Antibiotic resistant Escherichia coli in southeastern Australian pig herds and implications for surveillance

抗生素 抗生素耐药性 微生物学 生物 庆大霉素 多重聚合酶链反应 大肠杆菌 头孢菌素 肉汤微量稀释 抗菌剂 牧群 氨基糖苷 兽医学 医学 聚合酶链反应 基因 最小抑制浓度 遗传学 动物科学
作者
Lechelle K. Van Breda,Om P. Dhungyel,Michael P. Ward
出处
期刊:Zoonoses and Public Health [Wiley]
卷期号:65 (1) 被引量:25
标识
DOI:10.1111/zph.12402
摘要

Summary To investigate public health implications of antibiotics to control post‐weaning scours, we surveyed 22 commercial pig herds in southeastern Australia. Fifty faecal samples per herd were collected from pre‐ and post‐weaned piglets. Presumptive Escherichia coli isolates were confirmed by MALDI ‐ TOF MS . Isolates ( n = 325) were screened for susceptibility to 19 veterinary antibiotics using MIC broth microdilution. All 325 E. coli isolates underwent further testing against 27 antibiotics used in human medicine and were screened for ETEC adhesin and enterotoxin genes (F4 (K88), F5 (K99), F6 (987P), F18, F41, ST a, ST b, Stx2e and LT ) by multiplex PCR . Isolates identified as phenotypically resistant to third‐generation cephalosporin (3 GC ) and aminoglycoside antibiotics were screened by multiplex PCR /reverse line blot to detect common β‐lactam and aminoglycosides resistance genes, confirmed by sequencing. Twenty (6.1%) of the E. coli isolates were resistant to 3 GC antibiotics and 24 (7.4%) to the aminoglycoside antibiotic gentamicin. Genetic analysis revealed six different extended spectrum β‐lactamase ( ESBL ) genes ( bla CTX ‐M‐1, ‐14, ‐15, ‐27, bla SHV ‐12 and bla CMY ‐2‐like genes), four of which have not been previously reported in Australian pigs. Critically, the prevalence of 3 GC resistance was higher in non‐pathogenic (non‐ ETEC ) isolates and those from clinically normal (non‐diarrhoeal) samples. This highlights the importance of non‐ ETEC E. coli as reservoirs of antimicrobial resistance genes in piglet pens. Antimicrobial resistance surveillance in pig production focused on diagnostic specimens from clinically‐affected animals might be potentially misleading. We recommend that surveillance for emerging antimicrobial resistance such as to 3 GC antibiotics should include clinically healthy pigs.
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