生物
分子生物学
免疫电镜
基因表达
增殖细胞核抗原
基因
细胞生物学
遗传学
DNA
抗体
作者
Yasuhito Ide,Daisuke Tsuchimoto,Yoshihiro Tominaga,Yukihide Iwamoto,Yusaku Nakabeppu
出处
期刊:Genomics
[Elsevier]
日期:2003-01-01
卷期号:81 (1): 47-57
被引量:31
标识
DOI:10.1016/s0888-7543(02)00009-5
摘要
We isolated a mouse cDNA encoding APEX2 protein and demonstrated that APEX2 binds to PCNA. The level of Apex2 mRNA was high in the thymus, bone marrow, spleen, and kidney in adult mice. Apex2 consists of six exons and is flanked on the 3' end by Alas2 on X chromosome 63.0. Furthermore, Apex2 is flanked on the 5' end by a novel gene with a 106-bp intergenic sequence. We disrupted Apex2 in embryonic stem cells derived from a male mouse, and a 55-kDa APEX2 protein was detected in the nuclei of Apex2(+) but not Apex2-disrupted cells. Immunoelectron microscopy revealed that APEX2 is also localized in the mitochondria of Apex2(+) cells. In serum-stimulated BALB/c 3T3 cells, the level of Apex2 mRNA was transiently increased and the level of APEX2 reached a maximum in the late S phase, thus indicating that APEX2 may participate in postreplicative base excision repair.
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