亲和层析
抗体
化学
色谱法
串联亲和纯化
生物化学
生物
酶
免疫学
作者
Klaus Huse,H Böhme,Gerhard H. Scholz
出处
期刊:Journal of Biochemical and Biophysical Methods
[Elsevier]
日期:2002-05-01
卷期号:51 (3): 217-231
被引量:234
标识
DOI:10.1016/s0165-022x(02)00017-9
摘要
This review focusses on affinity purification of immunoglobulins, a methodology which is a powerful tool to obtain pure and intact antibodies. Affinity techniques allow antibody purification both in a single step chromatographic procedure as well as in complex purification protocols depending on the intention to use the target antibody. The purification strategies for antibodies by interaction with affinity ligands such as antibodies and Fe receptors or low molecular weight compounds are described.
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