Growth characteristics and phylogenetic analysis of the marine dinoflagellate Dinophysis infundibulus (Dinophyceae)

AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials AME 52:209-221 (2008) - DOI: https://doi.org/10.3354/ame01233 Growth characteristics and phylogenetic analysis of the marine dinoflagellate Dinophysis infundibulus (Dinophyceae) Goh Nishitani1, Satoshi Nagai1,*, Yoshihito Takano2, Sanae Sakiyama1, Katsuhisa Baba3, Takashi Kamiyama4 1Harmful Algal Bloom Division, National Research Institute of Fisheries and Environment of Inland Sea, 2-17-5 Maruishi, Hatsukaichi, Hiroshima 739-0452, Japan 2Faculty of Fisheries, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan 3Hokkaido Hakodate Fisheries Experimental Station, 1-2-66 Yunokawa, Hakodate, Hokkaido 042-0932, Japan 4Tohoku National Fisheries Research Institute, Fisheries Research Agency, 3-27-5 Shinhama, Shiogama, Miyagi 985-0001, Japan *Corresponding author. Email: snagai@affrc.go.jp ABSTRACT: We report the successful growth of the toxic dinoflagellate Dinophysis infundibulus under laboratory conditions, when fed the marine ciliate Myrionecta rubra grown with the cryptophyte Teleaulax amphioxeia; this study follows previous studies on D. acuminata, D. caudata, and D. fortii, showing the mixotrophy. We succeeded in maintaining several clonal strains of D. infundibulus for a relatively long period of time (>8 mo). Growth rates in D. infundibulus ranged from 0.40 to 0.94 divisions d–1, reaching a maximum concentration of 1.2 to 2.3 × 103 cells ml–1. The rate was 0.36 divisions d–1 when apparently fully expanded cells, resulting from the active ingestion of Myrionecta rubra, were cultivated without the ciliate prey. In contrast, the culture of D. infundibulus was not established in the absence of the ciliate or when provided only with the cryptophyte T. amphioxeia, suggesting that D. infundibulus cannot directly use T. amphioxeia as prey. We also determined the sequences of nuclear small subunit (SSU) rRNA, 5.8S rRNA with internal transcribed spacers (ITS1 and ITS2), and large subunit (LSU) rRNA regions in D. infundibulus. Phylogenetic relationships revealed that they are non-identical with regard to any of the DNA sequence data of Dinophysis deposited in GenBank, and are distinguishable from other Dinophysis species for species identification. KEY WORDS: Culture · Dinoflagellate · Dinophysis infundibulus · Myrionecta rubra · Teleaulax amphioxeia Full text in pdf format NextCite this article as: Nishitani G, Nagai S, Takano Y, Sakiyama S, Baba K, Kamiyama T (2008) Growth characteristics and phylogenetic analysis of the marine dinoflagellate Dinophysis infundibulus (Dinophyceae). Aquat Microb Ecol 52:209-221. https://doi.org/10.3354/ame01233Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in AME Vol. 52, No. 3. Online publication date: August 21, 2008 Print ISSN: 0948-3055; Online ISSN: 1616-1564 Copyright © 2008 Inter-Research.