Abstract Papers based biosensors such as lateral flow test strips and paper‐based microfluidic devices are inexpensive, rapid, flexible, and easy‐to‐use analytical tools for point‐of‐care diagnosis. An apparent trend in their detection is to interpret sensing results from qualitative assessment to quantitative determination. Electrochemical (EC) detection plays an important role in quantifying test results. This review focuses on paper‐based biosensors with EC detection. The first part provides detailed examples in lateral flow test strips while the second part gives an overview of paper microfluidics. The outlook and recommendation of future directions of papers based EC biosensors are discussed in the end.