Faster and easier chromatin immunoprecipitation assay with high sensitivity

Abstract Chromatin immunoprecipitation (ChIP) assays are widely used to investigate where chromatin‐binding proteins bind to the genome. The standard assay is very time consuming. We have developed a rapid ChIP assay in which the immunoprecipitates serve directly as PCR templates. This assay eliminates the step to reverse the crosslinking, shortening the assay by 1 day. It also requires a less immunoprecipitating antibody, permits many samples to be tested simultaneously, and is more sensitive than the standard ChIP assay.