Targeted metabolomics analysis reveals the association between maternal folic acid supplementation and fatty acids and amino acids profiles in rat pups

二十碳五烯酸 氨基酸 花生四烯酸 亚油酸 化学 新陈代谢 多不饱和脂肪酸 脂肪酸 脂肪酸代谢 亮氨酸 蛋氨酸 异亮氨酸 生物化学 亚麻酸
作者
Zhi‐Peng Liu,Rui Liu,Jing Chou,Jiaying Yu,Xiaowei Liu,Changhao Sun,Ying Li,Liyan Liu
出处
期刊:Journal of Chromatography B [Elsevier BV]
卷期号:1090: 101-109 被引量:10
标识
DOI:10.1016/j.jchromb.2018.05.013
摘要

Maternal diet during pregnancy can influence offspring's health by affecting development and metabolism. This study aimed to analyze the influence of maternal folic acid (FA) supplementation on the metabolism of rat pups using targeted metabolomics. Twenty female rats were randomly assigned to a FA supplementation (FAS group, n = 10) or control group (n = 10), which were fed AIN93G diet with 2 or 10 mg/kg FA, respectively. We then measured amino acids and their derivatives, biogenic amines, and fatty acids in the female rats and their pups by ultra-high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC/MS-MS) and gas chromatography-mass spectrometry (GC/MS-MS). In maternal rats, the significant changes of three metabolites (proline, γ-aminobutyric acid and esterified octadecatetraenoic acid, P < 0.05) were observed in FAS group. For the rat pups, FAS pups had significantly lower homocysteine and higher FA levels than control pups. The lower levels of amino acids (leucine, isoleucine, serine, proline) were obtained in FAS pups. Furthermore, there were the decreased esterified fatty acids (arachidonic acid, eicosapentaenoic acid, and docosatetraenoic acid) and free fatty acids (oleic acid, linoleic acid, γ-linolenic acid, octadecatetraenoic acid, arachidonic acid, eicosapentaenoic acid and selacholeic acid) in FAS pups. Metabolic changes in the FAS pups were characterized by changes in fatty acids and amino acids. These results suggested that FA supplementation during pregnancy influenced amino acids and fatty acids metabolism in rat pups. This study provides new insights into the regulation of amino acids and fatty acids metabolism during early life.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Ava应助Stardust采纳,获得10
1秒前
桐桐应助十九岁的时差采纳,获得10
1秒前
1秒前
2秒前
研究生完成签到,获得积分10
2秒前
自觉的凛发布了新的文献求助10
3秒前
所所应助开灯人和关灯人采纳,获得10
3秒前
讨厌科研发布了新的文献求助10
4秒前
快乐一江发布了新的文献求助10
4秒前
6秒前
Tianling完成签到,获得积分0
7秒前
好吃鱼完成签到,获得积分10
7秒前
等一个晴天完成签到,获得积分10
10秒前
胡涂涂发布了新的文献求助10
12秒前
研友_VZG7GZ应助小赞采纳,获得10
13秒前
量子星尘发布了新的文献求助10
14秒前
快乐一江完成签到,获得积分10
16秒前
17秒前
22秒前
啊标完成签到,获得积分10
22秒前
22秒前
水溶c100完成签到,获得积分10
23秒前
梨江鱼完成签到,获得积分10
23秒前
LcnTCM完成签到,获得积分10
23秒前
26秒前
maizhenpeng发布了新的文献求助10
27秒前
执着乐双发布了新的文献求助30
29秒前
小赞发布了新的文献求助10
29秒前
33秒前
37秒前
沈静完成签到,获得积分10
37秒前
39秒前
loski发布了新的文献求助10
39秒前
marina完成签到 ,获得积分20
40秒前
43秒前
时尚俊驰发布了新的文献求助10
43秒前
44秒前
文献发布了新的文献求助30
45秒前
48秒前
我嘞个豆完成签到,获得积分10
48秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989378
求助须知:如何正确求助?哪些是违规求助? 3531442
关于积分的说明 11254002
捐赠科研通 3270126
什么是DOI,文献DOI怎么找? 1804887
邀请新用户注册赠送积分活动 882087
科研通“疑难数据库(出版商)”最低求助积分说明 809173