人类受精
体外受精
精子
男科
体外
谷胱甘肽
化学
生物
细胞生物学
生物化学
胚胎
遗传学
医学
酶
作者
Toru Takeo,Naomi Nakagata
出处
期刊：CSH Protocols
[Cold Spring Harbor Laboratory]
日期：2018-04-18
卷期号：2018 (6): pdb.prot094524-pdb.prot094524
被引量：22
标识
DOI：10.1101/pdb.prot094524
摘要
The success of in vitro fertilization is strongly influenced by genetic background, handling of sperm and oocytes, and culture conditions. This protocol promotes enhanced rates of fertilization by using preincubation medium for sperm containing methyl-β-cyclodextrin (MBCD) and fertilization medium with high calcium and reduced glutathione (GSH).
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