Effects of Synthetic Peptides from HCV HVR1 on Cellular Immune Response Function.

Objective To study the effect of synthetic peptides(7-P) from HCV HVR1 on proliferation of mouse splenic lymphocytes in vitro.Methods Mice were immunized by 7-P through hypodermic injection or injected directly in the spleen before splenocytes were separated.After that,the splenocytes were stimulated again by 7-P in vitro,followed by MTS test to detect the effect of synthetic 7-P on splenocyte proliferation at different dose and incubation.Unimmunized mice were treated as negative control.Results 7-P could obviously promote the proliferation of splenocytes compared with control(without 7-P stimulation) and negative control groups(without 7-P immunization).Conclusion Synthetic peptides(7-P) derived from HCV HVR1 can promote splenocyte proliferation of 7-P-immunized mice in vitro.