STUDIES ON THE CHEMICAL CONSTITUENTS OF THE FLOWER OF CAMPSIS GRANDIFLORA (THUNB.)K.SCHUM.AND ITS CONTRACEPTIVE EFFECT

Chinese drugLing Xiao Hua,the dried flower of Campsis grandiflora (Thunb.)K.Schum.was specified as Flos Campsis in Chinese Pharmacopoeia(2000).Hentriacontanol(1),α ,β amyrin(2),β sitosterol(3),oleanolic acid(4),apigenin(5),cinnamic acid(6),15 mercapto 2 pentadecanone(7),daucosterol(8)and acteoside(9) were separated and identified by physiochemical and spectroscopic analyses.Besides β sitosterol,oleanolic acid and apigenin,the other 7 compounds were the first time separated from the dried flower of Campsis grandiflora (Thunb.)K.Schum.We also investigated the contraceptive effect of the five fractions of petroleum ether(60～90 ℃ PE),EtOAc(EA),Me 2CO(AC),Me 2CO∶CH 3OH(1∶1 AC∶ME=1∶1),and CH 3OH(ME),and three compounds,oleanolic acid,daucosterol,and acteoside.We found that EA,AC,AC∶ME(1∶1),and ME fractions (0.02 mg/ml)all can distinctly improve the contractive effect on the isolated pregnancy rat's uterine muscle( P 0.01).And AC,AC∶ME(1∶1) fraction had the most distinctly effect on improve the contractive strength of the isolated pregnancy rat's uterine muscle( P 0.01).