Irritancy and Allergic Responses Induced by Topical Application of ortho-Phthalaldehyde

戊二醛 刺激 敏化 局部淋巴结试验 免疫球蛋白E 淋巴结 人口 毒性 邻苯二甲醛 化学 免疫学 淋巴 男科 医学 抗体 病理 皮肤致敏 色谱法 高效液相色谱法 衍生化 有机化学 环境卫生
作者
Stacey E. Anderson,Christina Umbright,Rajendran Sellamuthu,Kara Fluharty,Michael L. Kashon,Jennifer Franko,Laurel G. Jackson,Victor J. Johnson,Pius Joseph
出处
期刊:Toxicological Sciences [Oxford University Press]
卷期号:115 (2): 435-443 被引量:37
标识
DOI:10.1093/toxsci/kfq054
摘要

Although ortho-phthalaldehyde (OPA) has been suggested as an alternative to glutaraldehyde for the sterilization and disinfection of hospital equipment, the toxicity has not been thoroughly investigated. The purpose of these studies was to evaluate the irritancy and sensitization potential of OPA. The EpiDerm Skin Irritation Test was used to evaluate in vitro irritancy potential of OPA and glutaraldehyde. Treatment with 0.4125 and 0.55% OPA induced irritation, while glutaraldehyde exposure at these concentrations did not. Consistent with the in vitro results, OPA induced irritancy, evaluated by ear swelling, when mice were treated with 0.75%. Initial evaluation of the sensitization potential was conducted using the local lymph node assay at concentrations ranging from 0.005 to 0.75%. A concentration-dependent increase in lymphocyte proliferation was observed with a calculated EC3 value of 0.051% compared to that of 0.089%, previously determined for glutaraldehyde. Immunoglobulin (Ig) E-inducing potential was evaluated by phenotypic analysis of draining lymph node (DLN) cells and measurement of total and specific serum IgE levels. The 0.1 and 0.75% exposed groups yielded significant increases in the IgE+B220+ cell population in the lymph nodes while the 0.75% treated group demonstrated significant increases in total IgE, OPA-specific IgE, and OPA-specific IgG1. In addition, significant increases in interleukin-4 messenger RNA and protein expression in the DLNs were observed in OPA-treated groups. The results demonstrate the dermal irritancy and allergic potential of OPA and raise concern about the proposed/intended use of OPA as a safe alternative to glutaraldehyde.
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