Anti-oxidative and anti-apoptosis effects of egg white peptide, Trp-Asn-Trp-Ala-Asp, against H2O2-induced oxidative stress in human embryonic kidney 293 cells

氧化应激 HEK 293细胞 活性氧 细胞凋亡 化学 二氯荧光素 分子生物学 活力测定 氧化磷酸化 生物化学 细胞生物学 生物 基因
作者
Jingbo Liu,Zhifei Chen,Jing He,Yan Zhang,Ting Zhang,Yiqun Jiang
出处
期刊:Food & Function [Royal Society of Chemistry]
卷期号:5 (12): 3179-3188 被引量:65
标识
DOI:10.1039/c4fo00665h
摘要

Peptides derived from egg white protein are well-known for their abundant species and biological properties. The aim of this study was to investigate the anti-oxidative and anti-apoptosis effects of (Trp-Asn-Trp-Ala-Asp) WNWAD, a pentapeptide derived from egg white ovomucin pepsin hydrolysates, against the oxidative stress induced by H2O2 in HEK-293 cells. Oxygen radical absorbance capacity (ORAC) results showed that WNWAD possessed extraordinary oxygen radical absorption capacity (with an ORAC value = 2.91 μM TE μM(-1)) in vitro. Then, at the cellular level, MTS assay results displayed that WNWAD dose-dependently inhibited H2O2-induced cellular oxidative stress in HEK-293 cells and fully recovered the oxidative damage induced by 400 μM H2O2 at a concentration of 1 μM. The 2',7'-dichlorofluorescein diacetate (DCFH-DA) fluorescent probe assay further proved that WNWAD inhibited cellular oxidative stress by reducing intracellular ROS accumulation in intact HEK-293 cells. In addition, scanning electron microscopy (SEM) images showed that the morphology of HEK-293 cells under H2O2 treatment displayed a cell apoptosis phenotype, while WNWAD pretreatment prevented the development of this phenotype. Furthermore, Western blot results indicated that WNWAD up-regulated the level of the anti-apoptotic protein Bcl-2 and down-regulated the levels of apoptosis executor proteins, cleaved caspase-3 and cleaved PRAP, in H2O2-induced oxidative stress in HEK-293 cells. All the above results suggested that WNWAD protected HEK-293 cells against H2O2-induced oxidative stress by inhibiting intracellular ROS accumulation and blocking the ROS activated mitochondria-mediated cell apoptosis pathway.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
万能图书馆应助虚影采纳,获得10
刚刚
严锦强发布了新的文献求助10
刚刚
酷波er应助qiu采纳,获得10
1秒前
飞快的尔芙完成签到,获得积分10
1秒前
囚徒发布了新的文献求助10
1秒前
脑洞疼应助小黑妞采纳,获得10
2秒前
niu完成签到,获得积分10
2秒前
2秒前
精明书包发布了新的文献求助10
3秒前
ding应助白日梦采纳,获得10
5秒前
小超超完成签到 ,获得积分10
5秒前
领导范儿应助不安太阳采纳,获得10
6秒前
万能图书馆应助周雪艳采纳,获得10
6秒前
6秒前
樱悼柳雪发布了新的文献求助80
6秒前
Harry发布了新的文献求助10
7秒前
Jasper应助kagami采纳,获得10
8秒前
MYYY完成签到,获得积分10
8秒前
8秒前
如意的忆秋完成签到,获得积分10
8秒前
lllllljmjmjm发布了新的文献求助10
8秒前
祖百川完成签到,获得积分10
8秒前
xuying158完成签到,获得积分10
9秒前
ypz完成签到,获得积分10
10秒前
Shauna完成签到,获得积分10
11秒前
香蕉觅云应助Kevin采纳,获得30
11秒前
11秒前
ldj6670发布了新的文献求助10
12秒前
iNk应助土坷垃采纳,获得20
12秒前
Ljc完成签到,获得积分10
13秒前
在水一方应助黑黑黑采纳,获得10
13秒前
研友_LX6YxZ完成签到,获得积分10
13秒前
HSY完成签到,获得积分10
13秒前
一亿完成签到,获得积分10
13秒前
少年锦时asd完成签到,获得积分10
14秒前
秦刚完成签到,获得积分10
14秒前
小二郎应助书文混四方采纳,获得10
15秒前
Erik完成签到,获得积分10
15秒前
16秒前
小鱼儿发布了新的文献求助10
16秒前
高分求助中
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
Effective Learning and Mental Wellbeing 300
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3974844
求助须知:如何正确求助?哪些是违规求助? 3519270
关于积分的说明 11197844
捐赠科研通 3255496
什么是DOI,文献DOI怎么找? 1797791
邀请新用户注册赠送积分活动 877187
科研通“疑难数据库(出版商)”最低求助积分说明 806202