Enantioconvergent hydrolysis of racemic 1,2-epoxypentane and 1,2-epoxyhexane by an engineered Escherichia coli strain overexpressing a novel Streptomyces fradiae epoxide hydrolase

大肠杆菌 拉伤 化学 弗氏链霉菌 立体化学 环氧化物水解酶 水解 链霉菌 环氧化物 生物转化 生物化学 催化作用 基因 细菌 放线菌 生物 解剖 遗传学 微粒体
作者
Rui Huang,Chuang Li,Shen Zhao,Qing-Tao Liu,Yan Liu,Zhenglian Xue
出处
期刊:Enzyme and microbial technology [Elsevier BV]
卷期号:166: 110228-110228 被引量:1
标识
DOI:10.1016/j.enzmictec.2023.110228
摘要

In order to excavate microbial epoxide hydrolases (EHs) with desired catalytic properties, a novel EH, SfEH1, was identified based on the genome annotation of Streptomyces fradiae and sequence alignment analysis with local protein library. The SfEH1-encoding gene, sfeh1, was then cloned and over-expressed in soluble form in Escherichia coli/BL21(DE3). The optimal temperature and pH of recombinant SfEH1 (reSfEH1) and reSfEH1-expressing E. coli (E. coli/sfeh1) were both determined as 30 ℃ and 7.0, also indicating that the influences of temperature and pH on reSfEH1's activities were more obvious than those of E. coli/sfeh1 whole cells. Subsequently, using E. coli/sfeh1 as catalyst, its catalytic properties towards thirteen common mono-substituted epoxides were tested, in which E. coli/sfeh1 had the highest activity of 28.5 U/g dry cells for rac-1,2-epoxyoctane (rac-6a), and (R)-1,2-pentanediol ((R)-3b) (or (R)-1,2-hexanediol ((R)-4b)) with up to 92.5% (or 94.1%) eep was obtained at almost 100% conversion ratio. Regioselectivity coefficients (αS and βR) displayed in the enantioconvergent hydrolysis of rac-3a (or rac-4a) were calculated to be 98.7% and 93.8% (or 95.2% and 98.9%). Finally, the reason of the high and complementary regioselectivity was confirmed by both kinetic parameter analysis and molecular docking simulations.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
儒雅致远发布了新的文献求助30
刚刚
刚刚
刚刚
yyy完成签到,获得积分20
1秒前
abala完成签到,获得积分10
1秒前
小二郎应助飞天817采纳,获得10
1秒前
Quinna发布了新的文献求助20
3秒前
yyy发布了新的文献求助30
4秒前
4秒前
abala发布了新的文献求助30
4秒前
郭小宝发布了新的文献求助10
4秒前
5秒前
科研通AI2S应助任性蘑菇采纳,获得10
5秒前
嘿嘿发布了新的文献求助10
5秒前
sundial发布了新的文献求助10
5秒前
6秒前
莫乞完成签到,获得积分10
6秒前
7秒前
我是老大应助木象爱火锅采纳,获得10
8秒前
9秒前
shuang0116发布了新的文献求助10
9秒前
ZJH发布了新的文献求助10
9秒前
10秒前
独角兽完成签到,获得积分10
10秒前
11秒前
NexusExplorer应助儒雅致远采纳,获得10
11秒前
chlgkmoney完成签到 ,获得积分20
11秒前
11秒前
12秒前
852应助科研通管家采纳,获得10
12秒前
wanci应助科研通管家采纳,获得10
12秒前
1111应助科研通管家采纳,获得20
12秒前
快乐的胖子应助科研通管家采纳,获得100
12秒前
12秒前
JamesPei应助科研通管家采纳,获得10
12秒前
skyer应助科研通管家采纳,获得10
12秒前
褪黑素应助科研通管家采纳,获得10
12秒前
CipherSage应助科研通管家采纳,获得10
12秒前
czh应助科研通管家采纳,获得10
12秒前
13秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Economic Geography and Public Policy 900
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3988786
求助须知:如何正确求助?哪些是违规求助? 3531116
关于积分的说明 11252493
捐赠科研通 3269766
什么是DOI,文献DOI怎么找? 1804771
邀请新用户注册赠送积分活动 881870
科研通“疑难数据库(出版商)”最低求助积分说明 809021