Detection of SARS-CoV-2 Spike Protein Using Micropatterned 3D Poly(3,4-Ethylenedioxythiophene) Nanorods Decorated with Gold Nanoparticles

The sensitivity and fabrication process of the detection platform are important for developing viral disease diagnosis. Recently, the outbreak of SARS-CoV-2 compelled us to develop a new detection platform to control such diseases in the future. We present an electrochemical-based assay that employs the unique properties of gold nanoparticles (AuNPs) deposited on 3D carboxyl-functionalized poly(3,4-ethylenedioxythiophene) (PEDOTAc) nanorods for specific and sensitive detection of SARS-CoV-2 spike protein (S1). The 3D-shaped PEDOTAc nanorods offer an ample surface area for receptor immobilization grown on indium–tin oxide surfaces through transfer-printing technology. Characterization via electrochemical, fluorescence, X-ray photoelectron spectroscopy, and scanning electron microscopy techniques confirmed the structural and morphological properties of the AuNPs-decorated PEDOTAc. In contrast to antibody-based assays, our platform employs ACE2 receptors for spike protein binding. Differential pulse voltammetry records current responses, showing linear sensitivity from 100 ng to 10 pg/mL of S1. In addition, the SARS-CoV-2 assay (CoVPNs) also exhibited excellent selectivity against nonspecific target proteins (H9N2, IL-6, and Escherichia coli). Furthermore, the developed surface maintained good stability for up to 7 consecutive days without losing performance. The results provide new insight into effective 3D conductive nanostructure formation, which is promising in the development of versatile sensory devices.