Functional characterization of RIP2 in large yellow croaker (Larimichthys crocea), a protein involved in the host antiviral responses via NF-κΒ, IRF3/7 related signaling

特里夫 IRF7 内部收益率3 细胞生物学 生物 信号转导衔接蛋白 信号转导 先天免疫系统 分子生物学 IκB激酶 磷酸化 NF-κB 生物化学 Toll样受体 受体
作者
Jing Jiang,Ying Li,Kai Qing Li,Ying Shen,Fang Li,Yi Lei Wang,Yong Jiang,Peng Fei Zou
出处
期刊:Fish & Shellfish Immunology [Elsevier BV]
卷期号:145: 109374-109374
标识
DOI:10.1016/j.fsi.2024.109374
摘要

As an adaptor protein functions essentially in the activation of NF-κΒ and MAPK signaling pathways mediated by NOD1 and NOD2, RIP2 plays important roles in the host innate immune responses. In the present study, the RIP2 ortholog termed Lc-RIP2 was identified and characterized in large yellow croaker (Larimichthys crocea). It was revealed that Lc-RIP2 is consisted of an open reading frame (ORF) of 1695 bp, encoding a protein of 564 aa, with an N-terminal kinase domain and a C-terminal caspase activation and recruitment domain (CARD). Subcellular localization assays demonstrated that Lc-RIP2 was a cytosolic protein, which was broadly distributed in the examined tissues/organs, and could be induced in response to poly I:C, LPS, PGN, and Pseudomonas plecoglossicida stimulations in vivo according to qRT-PCR analysis. Notably, Lc-RIP2 overexpression in vitro was sufficient to abolish SVCV proliferation in EPC cells, and could significantly induce the activation of NF-κB, IRF3, IRF7, and IFN1 promoters. In addition, luciferase assays found that Lc-RIP2 could cooperate with Lc-MAVS, Lc-TRAF3, Lc-TRAF6, Lc-IRF3, and Lc-IRF7 in NF-κB activation, associate with Lc-TRIF, Lc-MAVS, Lc-TRAF3, Lc-IRF3, and Lc-IRF7 in IRF3 activation, enhance Lc-TRIF, Lc-MAVS, Lc-TRAF3, and Lc-TRAF6 mediated IRF7 activation, and Lc-IRF3 mediated IFN1 activation, whereas suppress NF-κB activation when co-expressed with Lc-TRIF. Co-immunoprecipitation (Co-IP) assays also demonstrated that Lc-RIP2 interacts separately with Lc-TRIF, Lc-MAVS, Lc-TRAF3, Lc-TRAF6, Lc-IRF3, and Lc-IRF7. It is thus collectively indicated that Lc-RIP2 function dominantly in the regulation of the host innate immune signaling.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
枫叶的虫子完成签到,获得积分10
1秒前
1秒前
Pooh发布了新的文献求助10
2秒前
2秒前
3秒前
深情安青应助莫非采纳,获得10
4秒前
向阳而生o完成签到,获得积分10
4秒前
xxx发布了新的文献求助10
4秒前
5秒前
llll发布了新的文献求助10
6秒前
yao发布了新的文献求助30
6秒前
7秒前
loski发布了新的文献求助10
7秒前
可爱的函函应助偷乐采纳,获得10
9秒前
清晾油完成签到,获得积分10
9秒前
10秒前
脑洞疼应助科研通管家采纳,获得10
10秒前
科目三应助科研通管家采纳,获得10
10秒前
坦率的匪应助科研通管家采纳,获得10
10秒前
科研通AI5应助科研通管家采纳,获得10
10秒前
JamesPei应助科研通管家采纳,获得10
10秒前
解语花应助科研通管家采纳,获得50
10秒前
czh应助科研通管家采纳,获得10
10秒前
JamesPei应助科研通管家采纳,获得10
10秒前
科研通AI2S应助科研通管家采纳,获得10
10秒前
bkagyin应助科研通管家采纳,获得10
10秒前
Hello应助科研通管家采纳,获得10
10秒前
坦率的匪应助科研通管家采纳,获得10
10秒前
ludov应助科研通管家采纳,获得10
10秒前
11秒前
搜集达人应助科研通管家采纳,获得10
11秒前
隐形曼青应助科研通管家采纳,获得10
11秒前
坦率的匪应助科研通管家采纳,获得10
11秒前
11完成签到,获得积分20
11秒前
精明谷完成签到,获得积分10
11秒前
12秒前
12秒前
Liu完成签到,获得积分10
12秒前
bxkrystal发布了新的文献求助10
15秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3988838
求助须知:如何正确求助?哪些是违规求助? 3531250
关于积分的说明 11252914
捐赠科研通 3269838
什么是DOI,文献DOI怎么找? 1804820
邀请新用户注册赠送积分活动 881943
科研通“疑难数据库(出版商)”最低求助积分说明 809028