褐藻糖胶
化学
水解
多糖
化学结构
硫酸化
回转半径
褐藻
立体化学
生物化学
有机化学
藻类
植物
聚合物
生物
作者
Anh Ngoc Nguyen,Quang Van Ngo,Thu Thi Minh Quach,Suzuno Ueda,Yoshiaki Yuguchi,Yuki Matsumoto,Shinichi Kitamura,Cuong Duc Ho,Thuy Thi Thu Thanh
标识
DOI:10.1016/j.ijbiomac.2024.129326
摘要
The aims of this study are to determine the structure of a fucoidan from brown seaweed Turbinaria decurrens, to investigate its anticancer activity and structure-activity relationship. SEC-MALLS, IR, ESI-MS and NMR spectra analysis indicated that dominant structure of the fucoidan, with a Mw 122.6 KDa, has a backbone of (1 → 3)- and (1 → 4)-α-L-Fucp residues, branched at C-4, sulfate groups are attached at C-2, C-3 and C-4; branches are (1 → 4)-β-D-Galp residues and sulfated at C-2. The fucoidan was hydrolyzed by HCl aqueous solution to obtain hydrolyzed fucoidans. It is assumed that native and hydrolyzed fucoidans have a rod-like conformation in solution with cross-sectional radius of gyration (Rgc) ranged from 0.53 to 1.52 nm as estimated from SAXS measurements. The fucoidans show great anticancer activity against HT29 human colon cancer cell line with IC50 ranging from 5.41 ± 0.36 to 73.52 ± 2.54 μg/mL. Anticancer activity of the fucoidan could be significantly improved by lowering molecular weight, furthermore, fucoidan required small molecular weight, small molecular weight distribution and rod-like structure with a short branch length for high anticancer activity.
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