Immediate and lagged vegetation responses to dry spells revealed by continuous solar-induced chlorophyll fluorescence observations in a tall-grass prairie

Monitoring plants' responses to water deficit using remote sensing still faces large uncertainty, mostly due to the inaccurate characterization of plants' physiological responses. Solar induced chlorophyll fluorescence (SIF) contains information on plants' physiological processes which regulates the energy partitioning after solar radiation is absorbed by chlorophyll, providing new opportunities to monitor plant response to drought stress. However, the drought-induced physiological, biochemical, and structural changes are strongly coupled, hindering the mechanistic understanding of drought impacts on plants. Here, using tower-based observations of SIF together with high spectral resolution reflectance measurements, we derived the time series of the fraction of absorbed photosynthetically active radiation by canopy, chlorophyll content, and fluorescence efficiency using two radiative transfer model-based decomposition methods, and evaluated their responses to two consecutive dry spells at a tall-grass prairie site in the USA (34°59′05.0″ N, 97°31′20.6″ W). We observed a robust signal of afternoon depression based on the fluorescence efficiency estimates during the second dry spell, which had much lower soil moisture than the first one. The strong decline in fluorescence efficiency in the afternoon was likely caused by the high temperature and atmospheric dryness when the soil was dry. Such a direct physiological response contributed to 14.4% to 36.0% of seasonal variation of afternoon SIF, depending on the decomposition method used. Sustained water stress also caused lagged responses. Despite the subsequent rainfall after the dry spell, we observed a continued decline of SIF due to the lagged decline of chlorophyll content and green canopy coverage. Our study demonstrates the use of continuous SIF measurements to understand the development of drought effects on plants, and highlights the importance of afternoon SIF measurements for physiological stress detection.