三角线
化学
色谱法
烘烤
亲水作用色谱法
生咖啡
选择性
磷酰胺
咖啡豆
高效液相色谱法
有机化学
食品科学
物理化学
乐果
杀虫剂
农学
生物
催化作用
作者
Jianjun Zhong,Lingling Zhao,Yuanxin Guo,Zhanming Li,Hua Ye,Huanan Guan,Zhiqiang Xing,Weiqing Wang
标识
DOI:10.1016/j.jfca.2024.106011
摘要
The coffee phytochemical trigonelline and its roasting products nicotinic acid and N-methylpyridinium represent an important class of bioactives that mediate the health benefits of coffee consumption. However, there is a paucity of methods for their simultaneous analysis in coffee beans, especially those affordable and reliable for widespread routine use. To improve this situation, an easy-to-use HPLC−UV method was developed in this study based on a reversed-phase/cation exchange mixed-mode column showing unique chromatographic selectivity toward the polar and ionic analytes over the matrix components of coffee. The selectivity was achieved by using a decreasing acetonitrile gradient, which tuned the column to a cation-exchange/hydrophilic interaction (CEX/HILIC) mixed separation mode whilst minimizing its hydrophobic interaction capability. The method was assessed for figures of merit, giving limits of detection in coffee beans at around the 0.1 mg/100 g wet matter level, and verified for its applicability to a heterogeneous set of coffee bean samples differing in roasting degree and origin.
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