Integrated analysis and validation reveal CYTH4 as a potential prognostic biomarker in acute myeloid leukemia

髓系白血病 细胞周期 生物 癌基因 癌症 白血病 小桶 癌症研究 基因 肿瘤科 生物标志物 内科学 生物信息学 免疫学 基因表达 医学 遗传学 转录组
作者
Hong Wang,Yishu Xiao,Wei Zhou,Yonghui Li
出处
期刊:Oncology Letters [Spandidos Publishing]
卷期号:27 (3) 被引量:1
标识
DOI:10.3892/ol.2024.14236
摘要

Acute myeloid leukemia (AML) is a clonal hematological malignancy with high mortality rates. The identification of novel markers is urgent for AML. Cytohesins are a subfamily of guanine nucleotide exchange factors activating the ADP‑ribosylation factor family GTPases. While the important roles of cytohesins have been reported in various cancers, their function in AML remains unclear. The present study aimed to explore the prognostic impact of cytohesin‑4 (CYTH4) and the underlying molecular functions. RNA sequencing and AML clinical data were obtained from The Cancer Genome Atlas and Gene Expression Omnibus databases to investigate gene expression and survival. Using the R software, differentially expressed genes were identified between the high‑ and the low‑CYTH4 group. Functional enrichment analysis was conducted by Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, and Gene Set Enrichment Analyses. The CIBERSORTx tool was used to explore the proportions of different immune cell types. The molecular function of CYTH4 was also validated in vitro by examining cell growth, cell cycle, apoptosis and colony‑forming ability. CYTH4 was significantly upregulated in AML compared with other cancers and normal tissues. High CYTH4 expression was associated with high white blood count (P=0.004) and higher risk status (P<0.001). Patients with high CYTH4 expression had poor overall survival (OS; HR=2.19; 95% CI, 1.40‑3.44; P=0.0006; high vs. low) and event‑free survival (EFS; HR=2.32; 95% CI, 1.43‑3.75; P=0.0006; high vs. low), and these patients could benefit from transplantation (HR=0.29; 95% CI, 0.18‑0.47; P<0.0001; transplantation vs. chemotherapy). Multivariate analysis showed that high CYTH4 expression was independently associated with inferior OS (HR=2.49; 95% CI, 1.28‑4.83; P=0.007) and EFS (HR=2.56; 95% CI, 1.48‑4.42; P=0.001). Functional analysis showed that CYTH4 was involved in immunoregulation. In vitro validation showed knockdown of CYTH4 adversely affected cell growth and induced cell apoptosis, while overexpression of CYTH4 enhanced cell growth. Taken together, CYTH4 is expressed at high levels in AML and can potentially function as a prognostic biomarker.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
自觉的水瑶完成签到,获得积分10
1秒前
dd36完成签到,获得积分10
1秒前
无幻完成签到 ,获得积分10
2秒前
科研小白完成签到 ,获得积分10
2秒前
Akim应助junfeiwang采纳,获得10
2秒前
3秒前
turui完成签到 ,获得积分10
4秒前
共享精神应助灵巧的导师采纳,获得10
5秒前
sun完成签到,获得积分10
5秒前
刘三岁三岁完成签到,获得积分10
6秒前
科研通AI5应助DrTang采纳,获得10
6秒前
喵叽发布了新的文献求助10
6秒前
Ortho完成签到,获得积分10
7秒前
猪猪hero应助忧郁的猕猴桃采纳,获得10
7秒前
Owen应助子不语采纳,获得10
8秒前
MiriamYu完成签到,获得积分10
9秒前
10秒前
10秒前
努力的学完成签到,获得积分10
11秒前
美好颜发布了新的文献求助10
12秒前
Mingtiaoxiyue发布了新的文献求助10
13秒前
三年半完成签到,获得积分10
15秒前
15秒前
17秒前
背后海亦完成签到,获得积分10
18秒前
xiao发布了新的文献求助10
20秒前
qq发布了新的文献求助10
21秒前
快乐棒棒糖完成签到,获得积分10
21秒前
未完成发布了新的文献求助30
22秒前
DrTang发布了新的文献求助10
23秒前
所所应助小秋采纳,获得10
24秒前
小蘑菇应助科研通管家采纳,获得10
24秒前
衣吾余应助科研通管家采纳,获得10
25秒前
传奇3应助科研通管家采纳,获得10
25秒前
科研通AI5应助科研通管家采纳,获得10
25秒前
烟花应助科研通管家采纳,获得10
25秒前
25秒前
25秒前
25秒前
李健应助科研通管家采纳,获得10
25秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
Immigrant Incorporation in East Asian Democracies 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3965813
求助须知:如何正确求助?哪些是违规求助? 3511146
关于积分的说明 11156382
捐赠科研通 3245736
什么是DOI,文献DOI怎么找? 1793118
邀请新用户注册赠送积分活动 874230
科研通“疑难数据库(出版商)”最低求助积分说明 804268