姜黄素
抗氧化剂
线粒体生物发生
心磷脂
化学
生物化学
线粒体
功能(生物学)
细胞生物学
药理学
生物
膜
磷脂
作者
Xiaoting Sun,Yuanyuan Li,Yanqi Lin,Yuanyuan Mei,Lingli Lin,Kuo‐Ting Ho,Kaiyan Huang,Jialin Zhan,Chaoxiang Chen,Jun Zeng,Daren Wu,Jian Li,Jing Wang,Guiling Li
标识
DOI:10.1016/j.jff.2023.105541
摘要
The antioxidant activity of curcumin has been extensively investigated for years. However, its molecular function mechanism remained unclear. Our preliminary study showed that curcumin downregulated miR-22-3p expression. This study aimed to address whether miR-22-3p mediated the antioxidant activity of curcumin and the possible action mechanism. The results showed that miR-22-3p repression increased the total cellular antioxidant capacity and enhanced the endogenous antioxidant enzyme system of LO2 cells. In addition, miR-22-3p-deteriorated cellular antioxidant capacity was prevented by curcumin pretreatment. Moreover, miR-22-3p inhibition significantly improved mitochondrial function and biogenesis. Further investigation demonstrated that a mitochondrial key regulator, the cardiolipin synthase gene (CRLS1), was targeted and downregulated by miR-22-3p. CRLS1 overexpression also significantly improved cellular antioxidant capacity and enhanced mitochondrial function and biogenesis in LO2 cells. Taken together, all these data suggested that miR-22-3p modulated the antioxidant effect of curcumin via targeting CRLS1, which may provide novel insights into miRNA-mediated antioxidant mechanism of curcumin.
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