发酵产氢
暗发酵
发酵
基因组
制氢
氢
化学
生物化学
梭菌
糖酵解
生物制氢
基质(水族馆)
食品科学
生物
新陈代谢
细菌
基因
有机化学
生态学
遗传学
作者
Jinman Cao,Guilan Duan,Aijun Lin,Yaoyu Zhou,Siming You,Jonathan W.C. Wong,Guang Yang
标识
DOI:10.1016/j.biortech.2023.129080
摘要
Cu is widely present in the feedstocks of dark fermentation, which can inhibit H2 production efficiency of the process. However, current understanding on the inhibitory mechanisms of Cu, especially the microbiological mechanism, is still lacking. This study investigated the inhibitory mechanisms of Cu2+ on fermentative hydrogen production by metagenomics sequencing. Results showed that the exposure to Cu2+ reduced the abundances of high-yielding hydrogen-producing genera (e.g. Clostridium sensu stricto), and remarkably down-regulated the genes involved in substrate membrane transport (e.g., gtsA, gtsB and gtsC), glycolysis (e.g. PK, ppgK and pgi-pmi), and hydrogen formation (e.g. pflA, fdoG, por and E1.12.7.2), leading to significant inhibition on the process performances. The H2 yield was reduced from 1.49 mol H2/mol-glucose to 0.59 and 0.05 mol H2/mol-glucose upon exposure to 500 and 1000 mg/L of Cu2+, respectively. High concentrations of Cu2+ also reduced the rate of H2 production and prolonged the H2-producing lag phase.
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