生物传感器
电化学发光
清脆的
反式激活crRNA
检出限
化学
纳米技术
DNA
劈开
组合化学
材料科学
色谱法
生物化学
基因组编辑
基因
作者
Linying Yu,Yao Peng,Mengting Sheng,Qian Wang,Jianshe Huang,Xiurong Yang
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2023-07-04
卷期号:8 (7): 2852-2858
被引量:27
标识
DOI:10.1021/acssensors.3c00806
摘要
Rapid and accurate detection of biomarkers was very important for early screening and treatment of diseases. Herein, a sensitive and amplification-free electrochemiluminescence (ECL) biosensor based on CRISPR/Cas12a and DNA tetrahedron nanostructures (TDNs) was constructed. Briefly, 3D TDN was self-assembled on the Au nanoparticle-deposited glassy carbon electrode surface to construct the biosensing interface. The presence of the target would activate the trans-cleavage activity of Cas12a-crRNA duplex to cleave the single-stranded DNA signal probe on the vertex of TDN, causing the Ru(bpy)32+ to fall from the electrode surface and weakened the ECL signal. Thus, the CRISPR/Cas12a system transduced the change of target concentration into an ECL signal enabling the detection of HPV-16. The specific recognition of CRISPR/Cas12a to HPV-16 made the biosensor have good selectivity, while the TDN-modified sensing interface could reduce the cleaving steric resistance and improve the cleaving performance of CRISPR/Cas12a. In addition, the pretreated biosensor could complete sample detection within 100 min with a detection limit of 8.86 fM, indicating that the developed biosensor possesses the potential application prospect for fast and sensitive nucleic acid detection.
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