亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Virtual screening of quorum sensing inhibitors for Salmonella Typhimurium and their application as preservatives in chicken breast

群体感应 生物膜 沙门氏菌 化学 抗菌剂 食品科学 防腐剂 微生物学 食品防腐剂 生物 细菌 遗传学
作者
Zixuan Xu,Zhenyang Hu,Zhilong Yu,Lijun Huang,Fangwei Yang,Yunfei Xie
出处
期刊:Food bioscience [Elsevier BV]
卷期号:55: 102957-102957 被引量:7
标识
DOI:10.1016/j.fbio.2023.102957
摘要

Quorum sensing inhibitors (QSIs) have been used to inhibit the formation of biofilms produced by foodborne pathogens like Salmonella Typhimurium to improve food safety. However, conventional approaches for screening QSIs are time-consuming and yields random findings. In this study, virtual screening was applied to discover QSIs by targeting LuxS and LsrB, two quorum sensing-related proteins of Salmonella. Twelve QSIs that meet the use in the food industry were identified and their antimicrobial effects were further determined. Esculetin showing the lowest minimum inhibitory concentration (MIC, 500 μ g/mL) was demonstrated to be the most effective antimicrobial against Salmonella Typhimurium. Esculetin displayed significant inhibitory effect on the motility, biofilm formation, extracellular polymer and Autoinducer-2 signaling molecule production of Salmonella Typhimurium. The expression of adrA, lsrb, luxs and rpoS genes related to quorum sensing and biofilm formation was down-regulated by esculetin. This may be because esculetin and LsrB proteins compete to bind, thereby preventing the Autoinducer-2 signaling molecule from entering the membrane and disrupting quorum sensing behavior of Salmonella Typhimurium. After 30 min of treatment, 8 MIC of esculetin could kill 2 log CFU/mL of Salmonella Typhimurium. The study demonstrated that virtual screening employing LuxS and LsrB represented a novel strategy for identifying QSIs for Salmonella. Moreover, the findings indicated that esculetin possessed a quality preservation effect during chicken storage, thereby highlighting its potential as an alternative preservative in the food industry.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
李健的粉丝团团长应助kohu采纳,获得10
21秒前
qq完成签到,获得积分10
37秒前
思源应助Akitten采纳,获得30
42秒前
43秒前
46秒前
童大大发布了新的文献求助10
48秒前
淡漠发布了新的文献求助10
52秒前
廖庭毅完成签到,获得积分20
53秒前
汉堡包应助wen采纳,获得10
1分钟前
秋天完成签到,获得积分10
1分钟前
汉堡包应助科研通管家采纳,获得10
1分钟前
1分钟前
1分钟前
NexusExplorer应助Luo采纳,获得10
1分钟前
1分钟前
Luo发布了新的文献求助10
1分钟前
wintersss完成签到,获得积分10
1分钟前
2分钟前
Plum22发布了新的文献求助20
2分钟前
廖庭毅发布了新的文献求助10
2分钟前
2分钟前
2分钟前
山是山三十三完成签到 ,获得积分10
3分钟前
核桃发布了新的文献求助10
3分钟前
斯文败类应助科研通管家采纳,获得10
3分钟前
NexusExplorer应助科研通管家采纳,获得10
3分钟前
杨怂怂完成签到 ,获得积分10
4分钟前
4分钟前
4分钟前
朱朱子完成签到 ,获得积分10
5分钟前
Akim应助科研通管家采纳,获得10
5分钟前
GingerF应助科研通管家采纳,获得100
5分钟前
传奇3应助科研通管家采纳,获得10
5分钟前
5分钟前
5分钟前
5分钟前
Plum22发布了新的文献求助20
5分钟前
HAG完成签到,获得积分20
5分钟前
Perry完成签到,获得积分10
6分钟前
江枫渔火完成签到 ,获得积分10
6分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990105
求助须知:如何正确求助?哪些是违规求助? 3532119
关于积分的说明 11256456
捐赠科研通 3271016
什么是DOI,文献DOI怎么找? 1805171
邀请新用户注册赠送积分活动 882288
科研通“疑难数据库(出版商)”最低求助积分说明 809228