血管生成
微泡
内皮功能障碍
内皮干细胞
外体
Wnt信号通路
癌症研究
医学
生物
上皮-间质转换
细胞生物学
免疫学
小RNA
下调和上调
内分泌学
体外
信号转导
遗传学
基因
作者
Zizhong Hu,Jingfan Wang,Ting Pan,Xinsheng Li,Chao Tao,Yan Wang,Xingxing Wang,Zhengyu Zhang,Yu Liu,Weiwei Zhang,Xu Chen,Xinjing Wu,Qinyuan Gu,Fan Yang,Huiming Qian,Aime Mugisha,Songtao Yuan,Qinghuai Liu,Ping Xie
出处
期刊:Diabetes
[American Diabetes Association]
日期:2023-06-22
卷期号:72 (9): 1307-1319
被引量:1
摘要
Diabetic retinopathy (DR), one of the most common microangiopathic complications in diabetes, causes severe visual damage among working-age populations. Retinal vascular endothelial cells, the key cell type in DR pathogenesis, are responsible for abnormal retinal angiogenesis in advanced stages of DR. The roles of exosomes in DR have been largely unknown. In this study, we report the first evidence that exosomes derived from the vitreous humor of patients with proliferative DR (PDR-exo) promote proliferation, migration, and tube formation of human retinal vascular endothelial cells (HRVECs). We identified long noncoding RNA (lncRNA) LOC100132249 enrichment in PDR-exo via high-throughput sequencing. This lncRNA, also mainly derived from HRVECs, promoted angiogenesis both in vitro and in vivo. Mechanistically, LOC100132249 acted as a competing endogenous sponge of miRNA-199a-5p (miR-199a-5p), thus regulating the endothelial-mesenchymal transition promoter SNAI1 via activation of the Wnt/β-catenin pathway and ultimately resulting in endothelial dysfunction. In conclusion, our findings underscored the pathogenic role of endothelial-derived exosomes via the LOC100132249/miR-199a-5p/SNAI1 axis in DR angiogenesis and may shed light on new therapeutic strategies for future treatment of DR.This study provides the first evidence that exosomes derived from vitreous humor from patients with proliferative diabetic retinopathy participate in angiogenesis. The findings demonstrate an unreported long noncoding RNA (lncRNA), LOC100132249, by exosomal sequencing of vitreous humor. The newly found lncRNA LOC100132249, mainly derived from endothelial cells, promotes angiogenesis via an miRNA-199a-5p/SNAI1/Wnt/β-catenin axis in a pro-endothelial-mesenchymal transition manner.
科研通智能强力驱动
Strongly Powered by AbleSci AI