Elucidating the heterogeneity of immunotherapy response and immune-related toxicities by longitudinal ctDNA and immune cell compartment tracking in lung cancer

免疫疗法 免疫系统 肺癌 医学 肿瘤科 液体活检 临床试验 生物标志物 癌症免疫疗法 进行性疾病 T细胞 内科学 免疫学 疾病 癌症 生物 生物化学
作者
Joseph C. Murray,Lavanya Sivapalan,Karlijn Hummelink,Archana Balan,James R. White,Noushin Niknafs,Lamia Rhymee,Gavin Pereira,Nisha Rao,Jillian Phallen,Alessandro Leal,David L. Bartlett,Kristen A. Marrone,Jarushka Naidoo,Benjamin Levy,Samuel Rosner,Christine L. Hann,Susan C. Scott,Josephine Feliciano,Vincent K. Lam,David S. Ettinger,Qing Kay Li,Peter B. Illei,Kim Monkhorst,Blair A. Jobe,Robert B. Scharpf,Julie R. Brahmer,Victor E. Velculescu,Patrick M. Forde,Valsamo Anagnostou
标识
DOI:10.1101/2023.06.23.546338
摘要

Abstract Purpose Although immunotherapy is the mainstay of therapy for advanced non-small cell lung cancer (NSCLC), robust biomarkers of clinical response are lacking. The heterogeneity of clinical responses together with the limited value of radiographic response assessments to timely and accurately predict therapeutic effect -especially in the setting of stable disease-call for the development of molecularly-informed real-time minimally invasive predictive biomarkers. In addition to capturing tumor regression, liquid biopsies may be informative in evaluating immune-related adverse events (irAEs). Experimental design We investigated longitudinal changes in circulating tumor DNA (ctDNA) in patients with metastatic NSCLC who received immunotherapy-based regimens. Using ctDNA targeted error-correction sequencing together with matched sequencing of white blood cells and tumor tissue, we tracked serial changes in cell-free tumor load (cfTL) and determined molecular response for each patient. Peripheral T-cell repertoire dynamics were serially assessed and evaluated together with plasma protein expression profiles. Results Molecular response, defined as complete clearance of cfTL, was significantly associated with progression-free (log-rank p=0.0003) and overall survival (log-rank p=0.01) and was particularly informative in capturing differential survival outcomes among patients with radiographically stable disease. For patients who developed irAEs, peripheral blood T-cell repertoire reshaping, assessed by significant TCR clonotypic expansions and regressions were noted on-treatment. Conclusions Molecular responses assist with interpretation of heterogeneous clinical responses especially for patients with stable disease. Our complementary assessment of the tumor and immune compartments by liquid biopsies provides an approach for monitoring of clinical benefit and immune-related toxicities for patients with NSCLC receiving immunotherapy. Statement of translational relevance Longitudinal dynamic changes in cell-free tumor load and reshaping of the peripheral T-cell repertoire capture clinical outcomes and immune-related toxicities during immunotherapy for patients with non-small cell lung cancer.
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