极性(国际关系)
神经科学
海马结构
人口
生物
生物系统
细胞
生物化学
医学
环境卫生
作者
Madhuvanthi Kannan,Ganesh Vasan,Simon Haziza,Cheng Huang,Radosław Chrapkiewicz,Junjie Luo,Jessica A. Cardin,Mark J. Schnitzer,Vincent A. Pieribone
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2022-11-03
卷期号:378 (6619)
被引量:38
标识
DOI:10.1126/science.abm8797
摘要
Genetically encoded fluorescent voltage indicators are ideally suited to reveal the millisecond-scale interactions among and between targeted cell populations. However, current indicators lack the requisite sensitivity for in vivo multipopulation imaging. We describe next-generation green and red voltage sensors, Ace-mNeon2 and VARNAM2, and their reverse response-polarity variants pAce and pAceR. Our indicators enable 0.4- to 1-kilohertz voltage recordings from >50 spiking neurons per field of view in awake mice and ~30-minute continuous imaging in flies. Using dual-polarity multiplexed imaging, we uncovered brain state–dependent antagonism between neocortical somatostatin-expressing (SST + ) and vasoactive intestinal peptide–expressing (VIP + ) interneurons and contributions to hippocampal field potentials from cell ensembles with distinct axonal projections. By combining three mutually compatible indicators, we performed simultaneous triple-population imaging. These approaches will empower investigations of the dynamic interplay between neuronal subclasses at single-spike resolution.
科研通智能强力驱动
Strongly Powered by AbleSci AI