灰葡萄孢菌
采后
孢子萌发
生物
杀菌剂
几丁质酶
葡萄球菌炎
园艺
微生物学
生物化学
孢子
酶
作者
Wenbin Zhao,Jun‐Xia An,Yongmei Hu,An‐Ping Li,Shaoyong Zhang,Bao‐Qi Zhang,Zhijun Zhang,Xiong‐Fei Luo,Qiang Bian,Yue Ma,Yan‐Yan Ding,Rui Wang,Ying-Qian Liu
标识
DOI:10.1021/acs.jafc.2c03441
摘要
The inhibitory effect of tavaborole on the invasion of Botrytis cinerea in grapes and tomatoes, as well as the potential mechanism involved, was discovered in this study. Our findings showed that tavaborole inhibited Botrytis cinerea spore germination and mycelial expansion in vitro and that the control efficiency in vivo on fruit decay was dose-dependent, which was effective in reducing disease severity and maintaining the organoleptic quality of the fruit, such as reducing weight loss and retaining fruit hardness and titratable acid contents during storage. Furthermore, the precise mechanism of action was investigated further. Propidium iodide staining revealed that Botrytis cinerea treated with tavaborole lost membrane integrity. For further validation, cytoplasmic malondialdehyde accumulation and leakage of cytoplasmic constituents were determined. Notably, the inhibitory effect was also dependent on inhibiting the activities of aminoacyl-tRNA synthetases involved in the aminoacyl-tRNA biosynthesis pathway in Botrytis cinerea. The above findings concluded that tavaborole was effective against Botrytis cinerea infection in postharvest fruit, and a related mechanism was also discussed, which may provide references for the drug repurposing of tavaborole as a postharvest fungicide.
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