Quantitative detection of Epstein‐Barr virus DNA methylation in the diagnosis of nasopharyngeal carcinoma by blind brush sampling

鼻咽癌 采样(信号处理) DNA甲基化 聚合酶链反应 实时聚合酶链反应 病毒载量 CpG站点 医学 病理 生物 病毒学 病毒 内科学 基因 遗传学 基因表达 滤波器(信号处理) 计算机视觉 放射治疗 计算机科学
作者
Xiao‐Hui Zheng,Xi‐Zhao Li,Ting Zhou,Cheng‐Tao Jiang,Cao‐Li Tang,Changmi Deng,Ying Liao,Yong‐Qiao He,Tong‐Min Wang,Wei‐Hua Jia
出处
期刊:International Journal of Cancer [Wiley]
卷期号:152 (12): 2629-2638 被引量:8
标识
DOI:10.1002/ijc.34491
摘要

Abstract Detecting EBV DNA load in nasopharyngeal (NP) brushing samples for the diagnosis of nasopharyngeal carcinoma (NPC) has attracted widespread attentions. Currently, NP brush sampling mostly relies on endoscopic guidance, and there are few reports on diagnostic markers suitable for nonguided conditions (blind brush sampling), which is of great significance for extending its application. One hundred seventy nasopharyngeal brushing samples were taken from 98 NPC patients and 72 non‐NPC controls under the guidance of endoscope, and 305 blind brushing samples were taken without endoscopic guidance from 164 NPC patients and 141 non‐NPC controls (divided into discovery and validation sets). Among these, 38 cases of NPC underwent both endoscopy‐guided NP brushing and blind brushing. EBV DNA load targeting BamHI‐W region and EBV DNA methylation targeting 11029 bp CpG site located at Cp‐promoter region were detected by quantitative polymerase chain reaction (q‐PCR). EBV DNA load showed good classification accuracy for NPC in endoscopy‐guided brushing samples (AUC = 0.984). However, in blind bushing samples, the diagnostic performance was greatly reduced (AUC = 0.865). Unlike EBV DNA load, the accuracy of EBV DNA methylation was less affected by brush sampling methods, whether in endoscopy‐guided brushing (AUC = 0.923) or blind brushing (AUC = 0.928 in discovery set and AUC = 0.902 in validation set). Importantly, EBV DNA methylation achieved a better diagnostic accuracy than EBV DNA load in blind brushing samples. Overall, detection of EBV DNA methylation with blind brush sampling shows great potential in the diagnosis of NPC and may facilitate its use in nonclinical screening of NPC.
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