Relevance of mouse and human IBD patients-derived colon organoids to investigate intestinal macrophage differentiation

生物 免疫系统 类有机物 川地68 肠上皮 炎症性肠病 免疫学 炎症 巨噬细胞 结肠炎 胃肠道 溃疡性结肠炎 上皮 转录组 体外 细胞生物学 免疫组织化学 病理 医学 基因表达 疾病 生物化学 基因 遗传学
作者
Manoel Luís Costa,Muriel Pottier,Marie Paule Jacob,Pauline Zarnitzky,Benjamin Segain,Martin Figeac,Shéhérazade Sebda,Frédéric Leprêtre,Bertrand Meresse,Julie Demaret,Benoît Foligné,Annie Standaert–Vitse,Benjamin Bertin
出处
期刊:Journal of Leukocyte Biology [Oxford University Press]
标识
DOI:10.1093/jleuko/qiaf004
摘要

Abstract The gastrointestinal tract is a remarkable example of complex biology, with a constant dialogue between the intestinal epithelium, in close contact with the microbiota, and the immune cells that protect the gut from infection. Organoids have revolutionized our approach to modelling the intestinal cellular compartment and have opened new avenues for unravelling the mechanisms involved in intestinal homeostasis and chronic pathogenesis such as inflammatory bowel disease. To date, few models have been established to explore the role of the colon, which is however the main site of inflammation in ulcerative colitis (UC). Here, we used conditioned media produced by colon organoids (OCM) from mice or human (control and UC patients) to investigate the relationship between macrophages and the colon epithelium. We addressed transcriptomic profiles of OCM-stimulated bone marrow-derived macrophages and found that these cells exhibited a unique anti-inflammatory signature distinct from that of conventional in vitro IL-4/IL-13 M2 differentiated macrophages. In addition, OCM induced a clear CD5 antigen-like-mediated immunoregulatory effect characterized by a significant reduction in LPS-induced iNOS expression. In line, OCM from human colons inhibited LPS-dependent inflammatory cytokine expression in human monocytes-derived macrophages. Interestingly, the inflammatory marker CD68 was reduced by OCM from control patients but not from UC patients, suggesting epithelial dysfunction in UC patients. Our results report new regulatory mechanisms in the colon and highlight the importance of developing new in vitro models to better characterize the relationship between the intestinal epithelium and immune mucosal cells.
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