Discrimination between Purine and Pyrimidine-Rich RNA in Liquid–Liquid Phase-Separated Condensates with Cationic Peptides and the Effect of Artificial Crowding Agents

Membraneless organelles, often referred to as condensates or coacervates, are liquid-liquid phase-separated systems formed between noncoding RNAs and intrinsically disordered proteins. While the importance of different amino acid residues in short peptide-based condensates has been investigated, the role of the individual nucleobases or the type of heterocyclic structures, the purine vs pyrimidine nucleobases, is less researched. The cell's crowded environment has been mimicked