Oxidative stress induces release of mitochondrial DNA into the extracellular space in human placental villous trophoblast BeWo cells

氧化应激 抗霉素A 程序性细胞死亡 线粒体DNA 生物 线粒体 细胞生物学 细胞凋亡 细胞外 分子生物学 生物化学 基因
作者
Jennifer J. Gardner,Spencer C. Cushen,Reneé de Nazaré Oliveira da Silva,Jessica L. Bradshaw,Nataliia Hula,Isabelle K. Gorham,Selina Tucker,Zhengyang Zhou,Rebecca L. Cunningham,Nicole R. Phillips,Styliani Goulopoulou
出处
期刊:American Journal of Physiology-cell Physiology [American Physical Society]
标识
DOI:10.1152/ajpcell.00091.2024
摘要

Circulating cell-free mitochondrial DNA (ccf-mtDNA) is an indicator of cell death, inflammation, and oxidative stress. ccf-mtDNA differs in pregnancies with placental dysfunction from healthy pregnancies and the direction of this difference depends on gestational age and method of mtDNA quantification. Reactive oxygen species (ROS) trigger release of mtDNA from non-placental cells; yet it is unknown whether trophoblast cells release mtDNA in response to oxidative stress, a common feature of pregnancies with placental pathology. We hypothesized that oxidative stress would induce cell death and release of mtDNA from trophoblast cells. BeWo cells were treated with antimycin A (10-320 mM) or rotenone (0.2-50 mM) to induce oxidative stress. A multiplex real-time quantitative PCR (qPCR) assay was used to quantify mtDNA and nuclear DNA in membrane bound, non-membrane bound, and vesicular-bound forms in cell culture supernatants and cell lysates. Treatment with antimycin A increased ROS (p<0.0001), induced cell necrosis (p=0.0004) but not apoptosis (p=0.6471) and was positively associated with release of membrane-bound and non-membrane bound mtDNA (p<0.0001). Antimycin A increased mtDNA content in exosome-like extracellular vesicles (vesicular-bound form; p=0.0019) and reduced autophagy marker expression (LC3A/B, p=0.0002; p62, p<0.001). Rotenone treatment did not influence mtDNA release or cell death (p>0.05). Oxidative stress induces release of mtDNA into the extracellular space and causes non-apoptotic cell death and a reduction in autophagy markers in BeWo cells, an established in vitro model of human trophoblast cells. Intersection between autophagy and necrosis may mediate the release of mtDNA from the placenta in pregnancies exposed to oxidative stress.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
呼呼啦呼啦完成签到,获得积分10
1秒前
1秒前
Jasper应助sylnd126采纳,获得10
1秒前
哈哈发布了新的文献求助20
3秒前
Anita完成签到,获得积分10
3秒前
所所应助和谐一万采纳,获得10
4秒前
高有财完成签到 ,获得积分10
4秒前
4秒前
闪闪自中完成签到,获得积分10
5秒前
7秒前
jjym完成签到,获得积分10
8秒前
图南完成签到 ,获得积分10
8秒前
酷小裤完成签到,获得积分10
8秒前
9秒前
10秒前
项初蝶发布了新的文献求助10
10秒前
独特凡松发布了新的文献求助10
10秒前
10秒前
科研通AI5应助Luminous采纳,获得10
11秒前
傅寻菱完成签到,获得积分10
12秒前
方格发布了新的文献求助10
14秒前
14秒前
量子星尘发布了新的文献求助10
14秒前
14秒前
15秒前
16秒前
风清扬发布了新的文献求助10
16秒前
会发光的碳完成签到 ,获得积分10
16秒前
17秒前
17秒前
bkagyin应助斜玉采纳,获得10
17秒前
cookie发布了新的文献求助10
18秒前
笨小孩发布了新的文献求助200
18秒前
星辰大海应助苗条遥采纳,获得10
19秒前
赘婿应助kangkang采纳,获得10
19秒前
20秒前
冥月发布了新的文献求助10
21秒前
GGBOND完成签到,获得积分10
21秒前
是莉莉娅发布了新的文献求助10
22秒前
汉堡包应助哈哈哈大赞采纳,获得10
23秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3988732
求助须知:如何正确求助?哪些是违规求助? 3531027
关于积分的说明 11252281
捐赠科研通 3269732
什么是DOI,文献DOI怎么找? 1804764
邀请新用户注册赠送积分活动 881869
科研通“疑难数据库(出版商)”最低求助积分说明 809021