烟酰胺单核苷酸
烟酰胺
基质(水族馆)
化学
生物反应器
色谱法
生物化学
NAD+激酶
食品科学
酶
生物
烟酰胺腺嘌呤二核苷酸
有机化学
生态学
作者
Saroj Raj Kafle,Anamika Kushwaha,Lalit Goswami,Anoth Maharjan,Beom Soo Kim
标识
DOI:10.1016/j.biortech.2023.129911
摘要
Nicotinamide mononucleotide (NMN) subsists in all living organisms and has drawn tremendous attention as a nutraceutical and pharmaceutical product for several diseases such as Alzheimer's, cancer, aging, and vascular dysfunction. Here, NMN was produced intracellularly in a high cell density bioreactor using an engineered Escherichiacoli strain via exponential feeding of co-substrates. Fed-batch culture via exponential feeding of co-substrate (glucose) and continuous feeding of substrate (nicotinamide) were performed using different cumulative nicotinamide concentrations. The highest concentration of 19.3 g/L NMN with a dry cell weight of 117 g/L was acquired from a cumulative nicotinamide concentration of 7.2 g/L with a conversion of 98 % from nicotinamide in 28 h. Further, liquid chromatography-mass spectrometry analysis validated the NMN production. This approach will be beneficial in achieving simultaneously low cost and ensuring high quality and quantity of NMN production.
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