葛根素
GCLC公司
氧化应激
KEAP1型
GCLM公司
药理学
葛根
肝损伤
对乙酰氨基酚
化学
体内
抗氧化剂
生物化学
医学
生物
下调和上调
病理
替代医学
生物技术
转录因子
基因
作者
Wanhai Zhou,Heng He,Wei Qin,Lianqiang Che,Xin Zhao,Wenwen Liu,Yue Yan,Liangyu Hu,Yonghua Du,Zhongqiong Yin,Yong-Kang Shuai,Li Yang,Ruizhang Feng
摘要
Puerarin (Pue) is a kind of isoflavone compound extracted from Pueraria lobata, which has significant antioxidant activity. Excessive use of acetaminophen (APAP) can cause oxidative stress in the liver and eventually lead to acute liver injury. The purpose of this study was to investigate the protective effect and the mechanism of puerarin on APAP-induced liver oxidative damage. In in vitro experiments, puerarin significantly increased the cell activity of HepG2 cells, reduced the ROS accumulation, alleviated the oxidative damage and mitochondrial dysfunction. In in vivo studies, our results showed that puerarin enhanced antioxidant activity and alleviated histopathological damage. Further studies showed that puerarin decreased the expression of Keap1, promoted the nuclear migration of Nrf2, and up-regulated the expression of GCLC, GCLM, HO-1 and NQO1. This study demonstrated that puerarin can protect APAP-induced liver injury via alleviating oxidative stress and mitochondrial dysfunction by affecting the nuclear migration of Nrf2 via inhibiting Keap1.
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