Plasmonic Approach to Fluorescence Enhancement of Mesoporous Silica-Coated Gold Nanorods for Highly Sensitive Influenza A Virus Detection Using Lateral Flow Immunosensor

纳米棒 荧光团 材料科学 等离子体子 光漂白 纳米技术 荧光 介孔二氧化硅 检出限 介孔材料 光电子学 化学 光学 色谱法 物理 生物化学 催化作用
作者
Donggu Hong,Eun‐Jung Jo,Doyeon Bang,Chaewon Jung,Young Eun Lee,Yuseon Noh,Myung‐Geun Shin,Min‐Gon Kim
出处
期刊:ACS Nano [American Chemical Society]
卷期号:17 (17): 16607-16619 被引量:35
标识
DOI:10.1021/acsnano.3c02651
摘要

Rapid diagnostic tests based on the lateral flow immunoassay (LFI) enable early identification of viral infection, owing to simple interpretation, short turnaround time, and timely isolation of patients to minimize viral transmission among communities. However, the LFI system requires improvement in the detection sensitivity to match the accuracy of nucleic acid amplification tests. Fluorescence-based LFIs are more sensitive and specific than absorption-based LFIs, but their performance is significantly affected by fundamental issues related to the quantum yield and photobleaching of fluorophores. Metal-enhanced fluorescence (MEF), which is a plasmonic effect in the vicinity of metallic nanoparticles, can be an effective strategy to improve the detection sensitivity of fluorescence-based LFIs. The key factors for obtaining a strong plasmonic effect include the distance and spectral overlap of the metal and fluorophore in the MEF system. In this study, MEF probes were designed based on core-shell nanostructures employing a gold nanorod core, mesoporous silica shell, and cyanine 5 fluorophore. To optimize the efficiency of MEF probes incorporated on the LFI platform (MEF-LFI), we experimentally and theoretically investigated the distance dependence of plasmonic coupling between cyanine 5 and gold nanorods by adjusting the shell thickness, resulting in significant fluorescence enhancement. The proposed MEF-LFI enabled highly sensitive detection of influenza A virus (IAV) nucleocapsid protein with a detection limit of 0.52 pg mL-1 within 20 min and showed high specificity and accuracy for determining IAV clinical samples. Overall, our findings demonstrate the potential of this method as an effective tool for molecular diagnosis under emergency conditions.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
qq发布了新的文献求助10
刚刚
芦荟板蓝根完成签到,获得积分10
刚刚
柯一一应助科研通管家采纳,获得10
刚刚
星辰大海应助科研通管家采纳,获得10
刚刚
CodeCraft应助科研通管家采纳,获得10
1秒前
柯一一应助科研通管家采纳,获得10
1秒前
共享精神应助科研通管家采纳,获得10
1秒前
无花果应助科研通管家采纳,获得10
1秒前
yznfly应助科研通管家采纳,获得30
1秒前
Lucas应助科研通管家采纳,获得10
1秒前
萧水白应助科研通管家采纳,获得10
1秒前
SYLH应助科研通管家采纳,获得10
1秒前
赘婿应助科研通管家采纳,获得10
1秒前
田様应助科研通管家采纳,获得10
1秒前
JamesPei应助科研通管家采纳,获得10
2秒前
科目三应助科研通管家采纳,获得10
2秒前
2秒前
2秒前
Owen应助科研通管家采纳,获得30
2秒前
2秒前
2秒前
HAHA完成签到,获得积分10
3秒前
3秒前
善学以致用应助啊嘞嘞采纳,获得10
4秒前
啊啊完成签到,获得积分10
4秒前
干饭发布了新的文献求助10
5秒前
木木完成签到 ,获得积分10
6秒前
7秒前
8秒前
Vito发布了新的文献求助10
8秒前
afsdfds发布了新的文献求助10
9秒前
量子星尘发布了新的文献求助10
10秒前
ZXW完成签到,获得积分10
10秒前
11秒前
12秒前
nuonuoweng完成签到,获得积分10
12秒前
13秒前
小南发布了新的文献求助10
13秒前
kyt完成签到,获得积分10
14秒前
环游世界完成签到 ,获得积分10
14秒前
高分求助中
The Mother of All Tableaux Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 2400
Ophthalmic Equipment Market by Devices(surgical: vitreorentinal,IOLs,OVDs,contact lens,RGP lens,backflush,diagnostic&monitoring:OCT,actorefractor,keratometer,tonometer,ophthalmoscpe,OVD), End User,Buying Criteria-Global Forecast to2029 2000
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Cognitive Neuroscience: The Biology of the Mind (Sixth Edition) 1000
Optimal Transport: A Comprehensive Introduction to Modeling, Analysis, Simulation, Applications 800
Official Methods of Analysis of AOAC INTERNATIONAL 600
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3959547
求助须知:如何正确求助?哪些是违规求助? 3505776
关于积分的说明 11126213
捐赠科研通 3237706
什么是DOI,文献DOI怎么找? 1789252
邀请新用户注册赠送积分活动 871647
科研通“疑难数据库(出版商)”最低求助积分说明 802931