Fully Automatic Multi-Class Multi-Residue Analysis of Veterinary Drugs Simultaneously in an Integrated Chip-MS Platform

Microfluidic chip analysis has great potential advantages such as high integration, fast speed analysis, and automatic operation and is widely used not only in biological fields but also in many other analytical areas such as agriculture and food safety. Herein, a fully automatic multi-class multi-residue analysis of veterinary drugs simultaneously in an integrated chip-mass spectrometry (chip-MS) platform was developed. The developed microfluidic chip platform integrated three modules including the extraction and filtration module, "pass-through" clean-up module, and online evaporation module. The resulting chip has been coupled to a MS detector successfully, in which 23 kinds of residues in five classes were simultaneously qualitatively and quantitatively detected without chromatographic separation, obtaining the limits of detection of the spiked milk sample in the range of 0.23-4.13 ng/mL and the recovery rate in the range from 71.7 to 118.0% under optimized conditions. The microfluidic chip system developed in this study provided a new idea for the development of detection chips and exhibited considerable potential in the point-of-care testing in milk.