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The metabolic representation of ischemia in rat brain slices: A hyperpolarized 13 C magnetic resonance study

半影 灌注 乳酸脱氢酶 缺血 碳酸氢盐 医学 丙酮酸脱氢酶复合物 脑缺血 化学 磷酸肌酸 内科学 麻醉 内分泌学 生物化学 能量代谢
作者
David Shaul,Benjamin Grieb,Gal Sapir,Sivaranjan Uppala,Jacob Sosna,John M. Gomori,Rachel Katz‐Brull
出处
期刊:NMR in Biomedicine [Wiley]
卷期号:34 (7) 被引量:6
标识
DOI:10.1002/nbm.4509
摘要

The ischemic penumbra in stroke is not clearly defined by today's available imaging tools. This study aimed to develop a model system and noninvasive biomarkers of ischemic brain tissue for an examination that might potentially be performed in humans, very quickly, in the course of stroke triage. Perfused rat brain slices were used as a model system and 31 P spectroscopy verified that the slices were able to recover from an ischemic insult of about 3.5 min of perfusion arrest. This was indicated as a return to physiological pH and adenosine triphosphate levels. Instantaneous changes in lactate dehydrogenase (LDH) and pyruvate dehydrogenase (PDH) activities were monitored and quantified by the metabolic conversions of hyperpolarized [1‐ 13 C]pyruvate to [1‐ 13 C]lactate and [ 13 C]bicarbonate, respectively, using 13 C spectroscopy. In a control group (n = 8), hyperpolarized [1‐ 13 C]pyruvate was administered during continuous perfusion of the slices. In the ischemia group (n = 5), the perfusion was arrested 30 s prior to administration of hyperpolarized [1‐ 13 C]pyruvate and perfusion was not resumed throughout the measurement time (approximately 3.5 min). Following about 110 s of the ischemic insult, LDH activity increased by 80.4 ± 13.5% and PDH activity decreased by 47.8 ± 25.3%. In the control group, the mean LDH/PDH ratio was 16.6 ± 3.3, and in the ischemia group, the LDH/PDH ratio reached an average value of 38.7 ± 16.9. The results suggest that monitoring the activity of LDH and PDH, and their relative activities, using hyperpolarized [1‐ 13 C]pyruvate, could serve as an imaging biomarker to characterize the changes in the ischemic penumbra.
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