Development and Optimization of a Rapid Colorimetric Membrane Immunoassay for Porphyromonas gingivalis

牙龈卟啉单胞菌 免疫分析 格氏链球菌 微生物学 变形链球菌 抗体 慢性牙周炎 牙周炎 细菌 化学 分子生物学 生物 医学 链球菌科 免疫学 抗生素 内科学 遗传学
作者
Jiyon Lee,Myoung-Kwon Choi,Jinju Kim,Sechul Chun,Hong-Gyum Kim,HoSung Lee,Jin-Soo Kim,Dong­-Wook Lee,Sang Seop Han,Do‐Young Yoon
出处
期刊:Journal of Microbiology and Biotechnology [Springer Science+Business Media]
卷期号:31 (5): 705-709 被引量:2
标识
DOI:10.4014/jmb.2103.03029
摘要

Porphyromonas gingivalis (P. gingivalis) is a major bacterial pathogen that causes periodontitis, a chronic inflammatory disease of tissues around the teeth. Periodontitis is known to be related to other diseases, such as oral cancer, Alzheimer's disease, and rheumatism. Thus, a precise and sensitive test to detect P. gingivalis is necessary for the early diagnosis of periodontitis. The objective of this study was to optimize a rapid visual detection system for P. gingivalis. First, we performed a visual membrane immunoassay using 3,3',5,5'-tetramethylbenzidine (TMB; blue) and coating and detection antibodies that could bind to the host laboratory strain, ATCC 33277. Antibodies against the P. gingivalis surface adhesion molecules RgpB (arginine proteinase) and Kgp (lysine proteinase) were determined to be the most specific coating and detection antibodies, respectively. Using these two selected antibodies, the streptavidin-horseradish peroxidase (HRP) reaction was performed using a nitrocellulose membrane and visualized with a detection range of 103-105 bacterial cells/ml following incubation for 15 min. These selected conditions were applied to test other oral bacteria, and the results showed that P. gingivalis could be detected without crossreactivity to other bacteria, including Streptococcus mutans and Escherichia fergusonii. Furthermore, three clinical strains of P. gingivalis, KCOM 2880, KCOM 2803, and KCOM 3190, were also recognized using this optimized enzyme immunoassay (EIA) system. To conclude, we established optimized conditions for P. gingivalis detection with specificity, accuracy, and sensitivity. These results could be utilized to manufacture economical and rapid detection kits for P. gingivalis.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
1秒前
我要文献发布了新的文献求助10
2秒前
2秒前
2秒前
量子星尘发布了新的文献求助10
5秒前
暴躁小龙发布了新的文献求助10
6秒前
张润泽完成签到 ,获得积分10
6秒前
7秒前
文档发布了新的文献求助10
7秒前
8秒前
10秒前
11秒前
11秒前
12秒前
13秒前
13秒前
烟花应助zwk采纳,获得10
15秒前
蔡芝艳关注了科研通微信公众号
15秒前
YI完成签到,获得积分10
18秒前
18秒前
大个应助科研通管家采纳,获得10
19秒前
19秒前
充电宝应助科研通管家采纳,获得10
20秒前
SYLH应助科研通管家采纳,获得20
20秒前
Ava应助科研通管家采纳,获得10
20秒前
丘比特应助科研通管家采纳,获得10
20秒前
情怀应助科研通管家采纳,获得10
20秒前
Hello应助科研通管家采纳,获得10
20秒前
Owen应助科研通管家采纳,获得10
20秒前
20秒前
su发布了新的文献求助10
22秒前
钟垠州完成签到 ,获得积分10
22秒前
22秒前
活力的妙之完成签到 ,获得积分10
22秒前
丘比特应助只想困瞌睡采纳,获得10
22秒前
小宋发布了新的文献求助10
23秒前
笃定发布了新的文献求助30
25秒前
淡墨完成签到,获得积分10
26秒前
今后应助清漪采纳,获得10
26秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989297
求助须知:如何正确求助?哪些是违规求助? 3531418
关于积分的说明 11253893
捐赠科研通 3270097
什么是DOI,文献DOI怎么找? 1804884
邀请新用户注册赠送积分活动 882087
科研通“疑难数据库(出版商)”最低求助积分说明 809158